Cloning and Bioinformatics Analysis of TSP1 and TSP6 Gene of Echinococcus granulosus

doi:10.7668/hbnxb.2015.02.008

Abstract

Abstract: In order to study the function of two important antigen genes tetraspanin 1-TSP1(TSP1) and tetraspanin 1-TSP6(TSP6), primers derived from Echinococcus granulosus genome database in GenBank were designed and the open reading frame(ORF) sequences of TSP1 and TSP6 were cloned by RT-PCR from hydatid protoscolex.Then they were cloned into pMD19-T vector for bioinformatics analysis.The results indicated that the TSP1 cDNA contains 792 nucleotides.The deduced protein consisted of 263 amino acids and has three N-glycosylation sites, two N-acylation sites.The gene sequence showed about 98.99% identity with the TSP1 (EG_11043) reported and the induced amino acid sequence showed about 98.48% identity.The TSP6 cDNA contains 666 nucleotides.The deduced protein consisted of 221 amino acids and has five N-acylation sites, one Tyrosine kinase phosphorylation sites.The gene sequence showed about 98.18% identity with the TSP6 (EG_00715) reported and the induced amino acid sequence showed about 85.07% identity.The study carried out bioinformatics analysis of the TSP1 and TSP6 gene of Eg by molecular biology software to predict the structure and epitope of protein antigens known and laid a good foundation for the preparation of developing a vaccine.

Key words: Cystic echinococcosis, Antigen gene, Gene cloning, Bioinformatics analysis

CLC Number:

LIU Tian-li, MENG Qing-ling, QIAO Jun, CHEN Cheng, MA Yu, HU Zheng-xiang, CAI Xue-peng, CHEN Chuang-fu. Cloning and Bioinformatics Analysis of TSP1 and TSP6 Gene of Echinococcus granulosus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 41-46. doi: 10.7668/hbnxb.2015.02.008.

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