Construction and Primary Analysis of Tomato Leaf AD-cDNA Library Induced by Powdery Mildew Fungus

doi:10.7668/hbnxb.2015.02.009

Abstract

Abstract: In order to study the interaction between powdery mildew fungus and tomato, the AD-cDNA library of tomato leaves infected by Oidium neolycopersici was constructed using SMART technology.The titer of the constructed cDNA library was 3.77×10¹⁰ cfu, and the recombination rate of the library was about 96%, the insert fragments ranged from 400 bp to 2 000 bp, and the average length of the library was about 1 000 bp.Fifty clones were randomly picked from the library for sequencing and functional analysis.The results indicated that the library was qualified for the study on the interaction between powdery mildew fungus and tomato.

Key words: Tomato, Oidium neolycopersici, SMART technology, AD-cDNA library

CLC Number:

Q78
S436.412

LIU Yan, ZHANG Yi, KANG Jing-min, LIU Ke, ZHANG Chao, LI Peng-kun, AN Yi-bo, ZHOU Han, HUANG Hui, LI Cheng-wei. Construction and Primary Analysis of Tomato Leaf AD-cDNA Library Induced by Powdery Mildew Fungus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 47-50. doi: 10.7668/hbnxb.2015.02.009.

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