Construction of Yeast Two-hybrid cDNA Library of Verticillium dahliae

doi:10.7668/hbnxb.2015.02.010

Abstract

Abstract: To seek out the V.dahliae proteins involved in the interaction of plant with V.dahliae, a yeast two-hybrid library of V.dahliae was constructed using SMART technique.Results showed that the titer of cDNA library was 5.2×10⁷ cfu/mL, the library contained 3.9×10⁷ cfu independent clones, the size distribution of insert fragments ranged from 0.5-2.0 kb, and the recombination rate was about 96%.These data demonstrated that the library could meet the requirements of standard cDNA library, which laid a foundation for screening the interaction proteins from V.dahliae.

Key words: Verticillium wilt, Verticillium dahliae Kleb., Yeast two-hybrid cDNA library, SMART technique

CLC Number:

S432.1
Q78

KANG Jing-min, LIU Kun, LIU Yan, ZHANG Yi, LI Cheng-wei, TAN Guang-xuan. Construction of Yeast Two-hybrid cDNA Library of Verticillium dahliae[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 51-54. doi: 10.7668/hbnxb.2015.02.010.

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