The Mutations in the Promoter Region of CAPN4 and Its Effects on Carcass and Meat Quality Traits in Yak

doi:10.7668/hbnxb.2015.02.006

Abstract

Abstract: To search for the molecular markers associated with meat traits in yaks, CAPN4 mutations in the promoter region were investigated in different populations and their effects on carcass and meat quality were also analyzed.A total of eight hundred and thirty yaks which sourced from Gannan yak, Tianzhu white yak and Datong yak were used in this study.PCR-SSCP method was used to detect the CAPN4 mutations in the promoter region.Associations between mutations and the carcass and meat quality traits of Gannan yak were analyzed using the general linear model (GLM) in SPSS 19.0 program.The mutation (a.-1222G>A) in the promoter region was identified, which led to three PCR-SSCP patterns (AA, AB and BB).The results of the statistical analysis indicated that the genotypes and allele effected carcass and meat quality in Gannan yak with different ages.At the ages of four and six, yaks possessing the genotype BB had higher cooking rate than those with possessing the genotype AA and AB (P<0.05).At the ages of three and six, individuals with possessing the genotype AB had higher water loss rate than those with possessing the genotype BB and AA(P<0.05).However, yaks at the age of five, individuals with possessing the genotype BB had higher water loss rate than those with the genotype AA (P<0.05).At age of three and five, individuals with the genotype BB had bigger eye muscle area than those with possessing the genotype AA and BB (P<0.05).Yaks with possessing allele A had negative effect on cooking rate at the ages of four and six, as well as water-holding capacity and eye muscle area in all yaks.Excepting these yaks at the age of five, individuals with the presence of allele A had lower carcass weight (P<0.05), the mutation had no effects on carcass weight and tenderness.The mutation (a.-1222G>A) in the promoter of CAPN4 had an effect on carcass and meat quality partly, it might be used as a genetic marker to improve those traits in Gannan yak.

Key words: Yak, CAPN4 gene, Polymorphism, Carcass and meat quality traits

CLC Number:

Q78
S823.8+5

NIU Xiao-liang, LI Yan-qing, HU Jiang, LUO Yu-zhu, GUO Shu-zhen, YAN Wei, YANG Shu-meng. The Mutations in the Promoter Region of CAPN4 and Its Effects on Carcass and Meat Quality Traits in Yak[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 28-34. doi: 10.7668/hbnxb.2015.02.006.

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