Orthogonal Design and Optimization of SRAP Amplification System for Macrobrachium nipponense

doi:10.7668/hbnxb.2008.S2.027

Abstract

Abstract: Four factors ( Taq DNA polymerase,dNTP,Mg²⁺, primer) with four levels were studied by orthogonal design in order to establish SRAP technique of Macrobrachium nipponense. Firstly, effective amplification systems were screened out, which were optimized at the second step. Opt imal amplif icat ion system for Macrobrachium nipponense wasestablished with 2. 5 mmol/ L Taq DNA polymerase, 0.??25 mmol/ L dNTP, 0.64 U/ 20L Mg2+ and 0.6 mol/ L primer.The result laid on foundation for the application of SRAP marker in Macrobrachium nipponense.

Key words: Macrobrachium nipponense, Molecular marker, SRAP, Orthogonal design

CLC Number:

S945.1

YAO Jian-hua, FU Hong-tuo, GONG Yong-sheng, WU Yan, HE Xin-long, LI Hui-ping, QIAO Hui. Orthogonal Design and Optimization of SRAP Amplification System for Macrobrachium nipponense[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S2): 122-125. doi: 10.7668/hbnxb.2008.S2.027.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2008.S2.027

http://www.hbnxb.net/EN/Y2008/V23/IS2/122

[1]	WANG Jiarong, DONG Rui, ZHANG Mengyu, GAO Pu, ZHANG Peipei, LI Zaifeng, LIU Daqun. Identification of Leaf Rust Resistance Genes in 40 CIMMYT Wheat Lines at Seedling Stage and Adult Plant [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 201-210.
[2]	ZHANG Hui, WANG Liang, FU Zengjuan, LI Xiaodong, ZHAO Shangmin, E Yuanyuan, ZHENG Wenzhe, ZHANG Ziqiang, ZHANG Bizhou, ZHANG Huizhong. Application of VNTR-specific Primers in Fertility Identification of Sugar Beet [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 35-41.
[3]	LI Man, SHI Xiaolei, DI Rui, LIU Zhifang, MENG Qingmin, FU Cai, YANG Chunyan, WANG Dongmei, ZHANG Mengchen, ZHANG Jie, YAN Long. Development and Application of Large-size Insertion/Deletion Markers in Soybean [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 18-26.
[4]	WU Yue, CHEN Yang, WANG Xingzhe, SHAN Feibiao, ZHANG Yong, SUN Hongju. Transcriptome Sequencing and Bioinformatics Analysis of Astragalus complanatus [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 42-49.
[5]	ZHANG Hong, XU Yingli, WANG Chaonan, HUANG Zhiyin, FAN Weiqiang, LI Mei, ZHANG Bin. Development and Validation of Molecular Markers for Resistance to Dry Burning Heart Disease in Chinese Cabbage [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 195-203.
[6]	WU Xiaojun, LI Fengyuan, SHEN Yunxia, CHEN Xiangdong, HU Xigui, JIANG Hao, ZHANG Xuening, LI Xiaoli, HU Tiezhu, RU Zhengang. Molecular Marker Detection and Analysis of Allelic Variation Combinations of Grain Weight Genes Based on Introduced Wheat Germplasm Resources [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 127-134.
[7]	WU Tingting, CHEN Hailong, HUANG Jun, LIANG Yi, ZHANG Ting, LI Bowen, LAI Yifan, ZOU Yuying, CHE Fanhao, WU Jun, LIU Jinling, LIU Xionglun. *Improving Blast Resistance of CMS Line Fengyuan A by Pi9-based Marker-assisted Selection Strategy* [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 191-197.
[8]	GAO Guangliang, ZHANG Keshan, XU Guoyang, ZHAO Xianzhi, CHEN Zhuping, ZHOU Li, WANG Qigui. *Correlation Study on Polymorphism of GnIH* with Egg-Laying Traits and Body Measurement Traits in Goose** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 204-210.
[9]	WU Xiaojun, HU Xigui, CHEN Xiangdong, JIANG Xiaoling, ZHANG Xuening, WANG Yuquan, DONG Na, HU Tiezhu, LI Xiaohui, RU Zhengang. Detection and Practical Evaluation of Molecular Markers Associated with Drought Resistance Based on Introduced Wheat Germplasm Resources [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 74-82.
[10]	FENG Junyan, KANG Le, LANG Tao, ZHANG Cong, LI Ming, ZHAO Shan, PU Zhigang. Genetic Diversity Analysis of Sweet Potato and Its Wild Species Using SCoT Molecular Markers [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(1): 18-26.
[11]	NIU Xiaoxiao, LI Xiaobo, SUN Yi, MA Hailin, ZHANG Jie, LIU Yafei, HAO Yaoshan, CUI Guimei. Research Progress of Maize Stalk Rot Disease [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 433-440.
[12]	ZHANG Hao, LIU Xu, XUAN Ning, ZHANG Hua, GAO Ruiyu, ZHAO Qianqian, YAO Fangyin. Cultivation of Herbicide Resistance Rice with Low Amylose by Molecular Marker-assisted Selection [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 78-84.
[13]	MENG Yaning, YAN Libin, ZHANG Hongxiao, ZHAO Haitao, FAN Yanqin. *Screening and Detection of Resistance to Phytophthora* Blight Gene in Pepper Using Molecular Markers** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 338-341.
[14]	LIU Xiaodong, LI Haishan, MENG Chuan, WANG Yuhai, WU Fang, MU Jingui, MA Junxian, WANG Mingqiu. *A Rapid Identification Method and Application of a New Brassica napus* CMS in Chinese Cabbage** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(5): 26-31.
[15]	WANG Fei, ZHONG Xionghui, CHEN Denghui, LI Hailong, KANG Jungen, XIE Jianming. *Optimization of Protoplasts Preparation System and Establishment of Transient Transformation System in Brassica oleracea* var. capitata L.** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(3): 69-78.

Please choose a citation manager

Content to export