Effects of Shear Force on Microbial Transglutaminase Fermentation

doi:10.7668/hbnxb.2008.S2.028

Abstract

Abstract: The influences of glass beads on microbial transglutaminase fermentation in flask were studied. Effects of shear force on microbial transglutaminase fermentation were also studied in a 10 L fermenter by adjusting the agitation speed.The results showed that enhancing agitation speed has no significant effect on MTG act ivity at the duration of in?? oculum. MTG activity increased by 20% when four glass beads were put in the flask. Increasing shear force at 36 h would promote MTG synthesis. MTG act ivity and cell concentration boosted by 12% and 40% respect ively. In a 10 L fermenter, appropriate agitation speed is 250 r/ min, and MTG activity was increased by 21% via changing the agitation speed from 90 r/ min to 250 r/ min. Shear force affects MTG fermentat ion significantly.

Key words: Streptomyces sp. , Shear force, Transglutaminase, Glass bead

CLC Number:

FAN Xi-qiang, CHEN Yan-juan, SONG Min, ZHANG Ying-ying, CHANG Zhong-yi, GAO Hong-liang. Effects of Shear Force on Microbial Transglutaminase Fermentation[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S2): 126-128. doi: 10.7668/hbnxb.2008.S2.028.

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