Accumulation of Total Flavonoids at Reproductive Stage and Antioxidant Activity of Prunella vulgaris Linn．

doi:10.3969/j.issn.1000-7091.2012.02.032

Abstract

Abstract: The research was aimed to investigate the accumulation of total flavonoids in different organs and antioxidant activities in different extracts of Prunella vulgaris Linn．，which would provide theoretical basis for further?research． The total flavonoids contents in different organs during different periods were measured at reproductive?stage． Total flavonoids content was investigated by spectrometric method． The powder of P． vulgaris was extracted?with 70% ethanol (in water，v/v) using ultrasonic extraction，and then the filtrate was extracted followed with ethyl?acetate，n-butanol and water at equal volume． The extracts of different solvents could be gotten． The antioxidant activities of different extracts were evaluated and BHT was used as a positive control． The results showed that:the total?flavonoids content of roots，steams and leaves of P． vulgaris increased with plant growth and reached the peak at?flower-perished period，while that of the spikes reached the peak at flowers-blooming period． The total flavonoids?content of leaves was the highest one among all the organs． Scavenging abilities on DPPH· of the extracts and BHT?were in descending order:ethyl acetate ＞ n-butanol ＞ BHT ＞ water． Scavenging abilities on ·OH of the extracts and?BHT were in descending order:BHT ＞ ethyl acetate ＞ n-butanol ＞ water． There was strong correlation between antioxidant activity and total flavonoids content in different extracts of P． vulgaris． The flavonoids of P． vulgaris have?great antioxidant activity，and they can be obtained by processing the leaves or the whole herb which were collected?in flower-perished period or spike-dry period．

Key words: Prunella vulgaris Linn, Total flavonoids, Antioxidant activity , Free radical

CLC Number:

Q621.29

ZHANG Miao, BAI Yue-mei, MIAO Fang, ZHOU Le. Accumulation of Total Flavonoids at Reproductive Stage and Antioxidant Activity of Prunella vulgaris Linn．[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 170-174. doi: 10.3969/j.issn.1000-7091.2012.02.032.

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