Abstract: In this test,540 yellow catfish(Pelteobagrus fulvidraco) were selected,and fed with different levels of Ganoderma lucidum Polysaccharides(300,600,900,1 200,1 500 mg/kg diet,respectively) for 8 weeks.Then the head kidney macrophages and peripheral blood leukocytes of yellow catfish were separated by Percol1 continuous density gradient centrifugation.NBT reduction,Griesse reagent coloration and MTT assay were respectively used to evaluate the respiratory burst activity of head kidney macrophages and proliferation of peripheral blood leukocytes,in order to discuss the effects of GLP on the activation of immunological cells of yellow catfish.The results showed that: the activity of oxygen respiratory burst activity of head kidney macrophages and the activity of proliferation of peripheral blood leukocytes of every levels of GLP group were extremely enhanced(P0.01).Although the nitrogen respiratory burst activities of every level of GLP group were increased,only activities of 1 200-1 500 mg/kg GLP groups were obviously improved(P0.01).As a result,Ganodeima lucidum polysaccharides could effectively enhance the activation of immunological cells of the Yellow catfish.

Key words: Yellow catfish ( Pelteobagrus fulvidraco) , Ganoderma lucidum polysaccharides, Activation of immunological cells, Respiratory burest activity, Proliperation

CLC Number: 

• S963.736