Isolation and Identification of Nematophagous fungi and the Induction Effect of Amino Acids on It Trap Foming

doi:10.7668/hbnxb.2014.01.040

Abstract

Abstract: Nematode-trapping fungi were isolated with corn meal agar(CMA) from soils samples of Altay region in Xinjiang Province. 6 strains were successfully isolated and five strains identified as Arthrobotrys oligospora and the other one is Arthrobotrys conoides through morphological characteristics and molecular biology analysis. In addition, four amino acids(L-phenylalanine,L-tryptophan,L-methionine and L-valine) in different concentrations(0. 5, 0. 05,0. 005 g / L) were used as revulsive to induce the nematode-trapping organs forming of AS3 strain. The results showed that all the amino acids had effects on the traps forming,but the L-phenylalanine in concentration of 0. 05 g / L induced maximum number of trapping devices. This study laid a good foundation for biological prevention and control by nematode-trapping fungi.

Key words: Nematode-trapping fungus, Isolation and identification, Amino acids, Nematode-trapping devices forming

CLC Number:

S432.4

CHEN Shuang-qing, MENG Qing-ling, QIAO Jun, ZHAO Hai-long, WANG Jun-wei, WANG Wei-sheng, LUO Jian-xun, CAI Xue-peng. Isolation and Identification of Nematophagous fungi and the Induction Effect of Amino Acids on It Trap Foming[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 222-226. doi: 10.7668/hbnxb.2014.01.040.

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References

1 Gronvold J,Wolstrup J, Nansen P, et al. Nematode-trap-ping fungi against parasiteic cattle nematodes[J]. Parasi-tology Today,1993, 9 (4):137-140
2 Gronvold J,Hentiksen S A,Larsen M,et al. Biological con-trol;aspecas of hiological control with special reference toarthroopods,protozoan and helminthes of domesticated ani-coals [J]Veterinary Parasitology,1996, 64(1-2):47-64
3 Larsen M. The potential of nemaatophagous fungi to〔on-trol the free-living stages of nematode parasites of sheep: survey for the presence of fungi in fresh feces of grazing live stock in Australia[J]. Veterinary Parasitology,1994，53(3-5);275-281
4 孟庆玲，王为升，王俊伟，等少抱节丛抱菌的分离鉴定及捕食线虫活性研究[J]中国预防兽医学报，2011,33(3):189-193
5 Nordhring-Hertz B. Peptide-induced morphogenesis in the nematode trapping fungus Arthrobotrys oligo.spora[J] Physiologica Plantarum(Copenhagen)，1973，29(2): 223-233
6 Tumid A, Johansson T, Nordhring-Hertz B. Surface poly-mers of the nematode-trapping fungus Arthrobotrys oligo.s-pora[J]Journal of General Microhiology, 1991，137 (6):1231-1240
7 邓常胜，王瑞，杨晓野，等氨基酸对少抱节丛抱菌捕食器产生的影响[J]畜牧兽医学报，2009,40(4); 561-565
8 杨莲茹，杨晓野，杨玉琴，等捕食线虫真菌-少抱节丛抱菌CIMH1株的捕食特性研究[J]中国兽医寄生虫病，1999,7(2):14-17
9 White T J,BrunS T,Lee S,et al. Amplification and direca sequencing of fungal ribosomal RNA genes for phylogenet-ic[M]//Innis M A,Gelfand D A,Sninsky J J,eds. PCR Protocols; a guide to methods and applications. New York: Academic Press，1990;315-322
10 张宏真菌分类学中的分子生物学技术点评[J] 国外医学皮肤性病学分册，1997,22(4);215-219
11 张志华，洪葵核酸序列直接分析在真菌鉴定方面的应用[J]华南热带农业大学学报，2000,12(2); 39-43
12 王为升，孟庆玲，乔军，等新疆石河子地区捕食线虫性真菌的分离鉴定[J]中国兽医学报，2013,33 (2);230-235
13 Yang Y, Yang E C, An Z Q, et al. Evolution of nematode-trapping cells of predatory fungi of the Orbiliaceae based on evidence from rRNA-encoding DNA and multiprotein sequences[J]Proc Natl Acad Sci USA, 2007，104 (20);8379-8384

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