The Function Confirmation of Ve Gene Homolog in S. linnaeanum Related to Verticillium Wilt Resistance by VIGS

doi:10.7668/hbnxb.2014.01.039

Abstract

Abstract: Verticillium wilt is one of the most serious soil-borne diseases of eggplant. The exploration and utilization of Verticillium wilt resistant gene will play an important role for breeding disease resistant eggplants. Ve type genes have been confirmed to confer resistance to Verticillium wilt in some plant species. In this study,Ve homologous sequence was isolated from a Verticillium wilt resistant wild eggplant S. linnaeanum,and it was used to construct a recombinant vector TRV2-SlVe. The seedlings were inoculated with TRV2-SlVe mediated by Agrobacterium and followed Verticillium dahlia. The results showed that the infected plants expressed symptoms of Verticillium wilt, and the expression of Ve gene was down-regulated in leaf,stem and root by semi-quantitative RT-PCR. Above results indicate that the Ve gene homolog in S. linnaeanum confers resistance to Verticillium wilt.

Key words: S. Lauuaeauum, VIGS, Vertacallaum wilt, Ve

CLC Number:

S432.4

LIU Jun, ZHOU Xiao-hui, FENG Cui, WU Wen, ZHUANG Yong. The Function Confirmation of Ve Gene Homolog in S. linnaeanum Related to Verticillium Wilt Resistance by VIGS[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 217-221. doi: 10.7668/hbnxb.2014.01.039.

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