Isolation and Identification of Infectious Bursal Disease Virus and Partial Study of Its Characteristics

doi:10.7668/hbnxb.2010.05.010

Abstract

Abstract: Samples from a suspected chicken farm in Nanjing,Jiangsu province. With Clinical symptoms,pathological changes,artificial infection test,SPF embryo inoculation test,agar diffusion test and PCR amplification of special segment,the infection of infectious bursal disease virus was confirmed in this farm. A pure strain of infectious bursal disease virus was isolated. It was demonstrated that the isolated infectious bursal disease virus is a strongly virulent strain,which also shows good immunogenicity. Its physical and chemical characteristics were proved to be very similar with those of the standard strain of infectious bursal disease virus.

Key words: Infectious bursal disease virus, Isolation and identification, Sensitivity test, Immunogenicity

CLC Number:

S432.4+1

WANG Zhanwei, LIU MaojunFENG ZhixinXIONG QiyanWANG HaiyanBAI Yun, TANG YinghuaWU XusuGAN YuanLIU DongxiaWANG LiBAI Fangfang KONG MengWEI YannaLIU DongyangSHAO Guoqing1. Isolation and Identification of Infectious Bursal Disease Virus and Partial Study of Its Characteristics[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(5): 47-51. doi: 10.7668/hbnxb.2010.05.010.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2010.05.010

http://www.hbnxb.net/EN/Y2010/V25/I5/47

[1]	ZHANG Jian, WANG Na, LIU Zhida, BAO Tingting, ZHANG Zhiwei, YUN Xiaopeng, SHI Bixian, ZHANG Heng, CHEN Guihong, CHEN Yanfang, ZHAO Jun. Identification and Pathogenicity Differentiation of the Pathogens Causing Necrotic Spot on Sunflower Broomrape [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 172-180.
[2]	LIU Qingkun, FENG Hua, REN Chunxiao, WEI Qiang, LIU Yunchao, ZHANG Gaiping. The Impact of Different B Cell Epitopes on PCV2 Cap's Immunogenicity after Conjugated at C-terminal of Cap [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(3): 233-238.
[3]	LI Chen, WANG Siyue, JI Shaoze, LI Xuesong, ZHANG Xiqing, TIAN Jiaqi, MA Hongxia, GAO Yunhang. Isolation, Identification, Drug Sensitivity Test and Detection of Drug Resistance Genes of Bovine Acinetobacter calcoaceticus [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(S1): 318-323.
[4]	GUO Wenbo, HA Fu, JIANG Mingxing, ZHANG Zifang, QIAN Lindong, BAO Zhipeng, MIAO Yongwang. *Isolation, Identification, Tissue Expression and Bioinformatics Analysis of BMP4* Gene from Binlangjiang Buffalo** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(4): 223-230.
[5]	LI Chen, WANG Siyue, JI Shaoze, ZHANG Xiqing, LI Xuesong, TIAN Jiaqi, MA Hongxia, GAO Yunhang. Isolation,Identification,Drug Resistance and Detection of Macrolide Resistance Gene of Sow Moraxella osloensis [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(S1): 54-58.
[6]	XU Siyao, HUANG Pu, LI Ping, YANG Fan, JIANG Ziyi, LÜ Wenting, XU Zhiwen, ZHU Ling. *Antimicrobial Susceptibility and SCCmec* Genotyping of Methicillin Staphylococcus aureus Isolates from Swine in Sichuan** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(S1): 23-29.
[7]	CHEN Jin, HUANG Chunjuan, YU Xiaoming, HOU Liting, QIAO Xuwen, ZHENG Qisheng, HOU Jibo. *Screening the Immune Enhancer of Porcine epidemic diarrhea virus* Subunit Vaccine** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(2): 205-210.
[8]	YU Xiao-ming, CHEN Jin, HOU Li-ting, WU Nan, HOU Ji-bo, ZHENG Qi-sheng. *Isolation,Identification and Virulence Analysis of Porcine Epidemic Diarrhea Virus* TX Strain** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 342-347.
[9]	ZHANG Li, KANG Xue-yan, ZHANG Zhen-hua, LI Yong-qing, GAI Li-li, JIANG Shi-jin, ZHANG Pei-jun. Expression and Immunogenicity Analysis of Spike Glycoprotein of Transmissible Gastroenteritis Virus in Yeast Pichia pastoris [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(6): 84-90.
[10]	ZHANG Feng-feng, XIE Feng-xing, ZHOU Ke, SUN Hai-bo, ZHAO Yu-jie. Isolation and Identification of One Bacterium Species with High Effective Ability of Removing Ammonia-nitrogen [J]. journal1, 2014, 29(4): 221-225.
[11]	CHEN Shuang-qing, MENG Qing-ling, QIAO Jun, ZHAO Hai-long, WANG Jun-wei, WANG Wei-sheng, LUO Jian-xun, CAI Xue-peng. *Isolation and Identification of Nematophagous fungi* and the Induction Effect of Amino Acids on It Trap Foming** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 222-226.
[12]	WANG Xiao-min, HE Kong-wang, ZHOU Zhong-tao, MAO Ai-hua, YU Zheng-yu, WANG Wei, NI Yan-xiu, WEN Li-bin, ZHANG Xue-han, GUO Rong-li, LI Bin, YU Yang. Isolation,Identification and Genetic Variation of Porcine Reproductive and Respiratory Syndrome Virus Variant [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 232-238.
[13]	JIA Qiu-lei, WANG Lin, CHEN Peng, YANG Qing, JIANG Xiu-ping. *Homology Modeling and B Cell Antigenic Epitopes Predicition of Defibrase from Agkistrodon hadys ussuriensis* Snake Venom** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(S1): 97-102.
[14]	GUO Rong-li, NI Yan-xiu, WEN Li-bin, LI Bin, WANG Xiao-min, YU Zheng-yu, HE Kong-wang. Isolation and Identification of Porcine Transmissible Gastroenteritis Virus JS2012 Strain and Clone and Sequence Analysis of Its S Complete Genome [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(5): 74-79.
[15]	Zhan Lie, Lu Bingyang, Liu Huadong, Wang Caixian, Tang Juan, Zhan Haijie1, Li Qin, Wang Jianzhong, Ding Zhuang. Molecular Identification of Very Virulent Infectious Bursal Disease Virus [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 234-238.

Please choose a citation manager

Content to export