Plant Expression Vector Construction of Apple PGIP Gene and Its Transformation into Tomato

doi:10.7668/hbnxb.2010.05.009

Abstract

Abstract: Aimed to study the function of polygalacturonase-inhibiting protein gene in plant,breed disease resistant cultivar,obtain the transgenic plant,a plant expression vector pWR306-PGIP was constructed by inserting the PGIP gene coding fragment from cloning vector pMD-18T by SalⅠand BamHⅠsites into pWR306 between of ED35s promotor and NOS terminator. The identification results showed that the plant expression vector pWR306-PGIP and Agrobacterium tumefaciems were constructed successfully. Subsequently PGIP gene was transformed into Zhongshu No. 4 tomato leaves and shoot fragments mediated by Agrobacterium tumerfaciens. Eight transgenic resistant plants were proved by PCR preliminarily that the PGIP gene had integrated into tomato genome

Key words: PGIP gene, Plant expression vector, Agrobacterium tumerfaciens, Genetic transformation, Tomato

CLC Number:

SHI Yu, ZHANG Junke, ZHANG Yi, HONG Xiaojuan, MA Fengwang. Plant Expression Vector Construction of Apple PGIP Gene and Its Transformation into Tomato[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(5): 42-46. doi: 10.7668/hbnxb.2010.05.009.

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