Using PCR Product to Establish a Binary RNAi Transgenic Construct

doi:10.7668/hbnxb.2010.05.011

Abstract

Abstract: RNAi has been widely used in plant genetic improvement and gene function analysis in recent years. The conventional way to construct the hairpin structure of RNAi binary vector is based on the restriction enzyme digestion and ligation. However,this method is labour intensive and time consumption. In this study,we established a binary transgenic vector with PCR product,which habouring Hpt resistant gene and driving by constitutive promoter,and which would facilitate further rice transformation.

Key words: RNAi, Binary Construct, PCR product

CLC Number:

YANG JieZHONG, WeigongWANG, CailinLIU Qing, WANG Mingbo, Narayana M. Upadhyaya. Using PCR Product to Establish a Binary RNAi Transgenic Construct[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(5): 52-55. doi: 10.7668/hbnxb.2010.05.011.

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http://www.hbnxb.net/EN/Y2010/V25/I5/52

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