Transformation of GhACT1 RNAi and Its Effect on Fiber Length of Cotton(Gossypium hirsutum)

doi:10.7668/hbnxb.2008.05.016

Abstract

Abstract: Introduced the actin RNAi(interference)plasmid PBI121 GhACT1 into Coker 312 by Agrobacterium mediate method,and abtained the regenerants through somatic embryogenesis.NPTII as probes to hybridize,Southern result showed that there were two copies inserted into A5,A6 and A14 transgenic lines,the other six lines had multiple copies insertion.That declared these lines were transgenic positive lines.Observed under microscope,the young fiber on RNAi transgenic seeds was 3-5 fold shorter than that on tissue culture immature seeds.Analysis mature fiber length by statistic F test,result showed that F value beyond F_0.05=4.10,the fiber length of RNAi transgenic lines was significant shorter than tissue cultural fiber.This declared that the GhACT1 gene played a significant role in fiber length,and function mainly at early elongation period.

Key words: RNAi construct, Actin, Transformation, Fiber length

CLC Number:

S562.05

FAN Xiao-ping, FAN Bo-hong, LI Xue-bao, YANG Wei-cai, XU Zi-qin. Transformation of GhACT1 RNAi and Its Effect on Fiber Length of Cotton(Gossypium hirsutum)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(5): 73-75. doi: 10.7668/hbnxb.2008.05.016.

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