Identification of Interacting Proteins of Resistance-related Gene T1N6_22 in Arabidopsis thaliana by Yeast Two-hybrid

doi:10.7668/hbnxb.2018.02.001

Abstract

Abstract: The objective of this study is to identify interacting proteins of resistance-related gene T1N6_22 in Arabidopsis thaliana, and to provide a basis for clarifying the regulation mechanism of the T1N6_22 gene in Arabidopsis resistance. The yeast two-hybrid vectors of the T1N6_22 gene and its candidate interacting protein gene,including AD-T1N6_22, AD-AT1G06050, AD-AT1G21400, AD-AT2G19480, BD-T1N6_22, BD-AT1G06050, BD-AT1G21400, and BD-AT2G19480 were constructed by Gateway method. In order to detect self-activation of T1N6_22, AT1G06050,AT1G21400, and AT2G19480 genes,the AD vector was combined with BD-T1N6_22, BD-AT1G06050, BD-AT1G21400, and BD-AT2G19480 respectively,and transformed into yeast competent cells. The results showed that AT1G06050 had self-activation activity and T1N6_22, AT1G21400, AT2G19480 didn't have self-activation activity. For yeast two hybrid, the AD-T1N6_22 vector was combined with BD-AT1G06050, BD-AT1G21400, BD-AT2G19480 vectors,respectively, and the BD-T1N6_22 vector was combined with AD-AT1G06050, AD-AT1G21400, AD-AT2G19480 vectors,respectively. It was found that yeast colonies which were co-transformed with AD-T1N6_22 and BD-AT1G06050, BD-AT1G21400 or BD-AT2G19480, BD-T1N6_22 and AD-AT1G06050, AD-AT1G21400 or AD-AT2G19480 grew well in both -Leu/-Trp and -Leu/-Trp/-His and -Leu/-Trp/-His/-Ade media.Yeast two-hybrid results indicated that the T1N6_22 gene directly interacted with AT1G06050,AT1G21400 and AT2G19480 in yeast.

Key words: Arabidopsis thaliana, T1N6_22, Yeast two-hybrid, Interacting protein

CLC Number:

Q945.8
Q78

ZHAO Yating, XING Hongxia, PANG Xi, ZHENG Xu, ZHANG Jing, WENG Qiaoyun, XING Jihong, DONG Jingao. Identification of Interacting Proteins of Resistance-related Gene T1N6_22 in Arabidopsis thaliana by Yeast Two-hybrid[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(2): 1-7. doi: 10.7668/hbnxb.2018.02.001.

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