Screening and Analysis of Interacting Proteins of T1N6_22 Gene from Arabidopsis Against Botrytis cinerea

doi:10.7668/hbnxb.2017.02.007

Abstract

Abstract: Botrytis susceptible mutant named T1N6_22 was achieved from Arabidopsis mutant library.Through the method of TAIL-PCR,resistance-related gene T1N6_22 of Arabidopsis thaliana against Botrytis cinerea infection was cloned.In order to further clarify the regulation mechanism of T1N6_22 gene in Arabidopsis thaliana against Botrytis cinerea infection,vector pAS1/T1N6_22 of T1N6_22 gene was constructed.Taking pAS1/T1N6_22 fusion protein as bait, Arabidopsis cDNA library were screened by the method of yeast two-hybrid system.A total of 28 yeast clones were obtained by yeast two hybrid screening.The positive clones were identified by T1N6_22 gene specific primers and sequenced.Four interacting proteins (AT2G19480,AT1G06050,AT5G34780 and AT1G21400)were obtained by screening Arabidopsis cDNA library.Blast analysis showed that four interacting proteins encoded nucleosome assembly protein,function unknown protein,putative ketopantoate reductase,and oxidoreductase,respectively.These interacting proteins were related to process,biosynthesis of pantothenate,and plant metabolic process.These results would provide foundation for identifying interacting protein of T1N6_22 and clarifying the regulation mechanism of T1N6_22 gene in Arabidopsis resistance.

Key words: Arabidopsis, T1N6_22, Yeast two-hybrid, Interacting protein

CLC Number:

Q78
S432

WENG Qiaoyun, HUANG Congcong, WANG Na, LIANG Chenxi, LIU Gaoran, HAO Congcong, XING Jihong, DONG Jingao. Screening and Analysis of Interacting Proteins of T1N6_22 Gene from Arabidopsis Against Botrytis cinerea[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(2): 45-49. doi: 10.7668/hbnxb.2017.02.007.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2017.02.007

http://www.hbnxb.net/EN/Y2017/V32/I2/45

References

[1] Keller M,Viret O,Cole F M. Botrytis cinerea infection in grape flowers:Defense reaction,latency,and disease expression[J].Phytopathology,2003,93(3):316-322.
[2] Zhu B,Zhou Q,Xie G,et al.Interkingdom gene transfer may contribute to the evolution of phytopathogenicity in Botrytis cinerea[J].Evolutionary Bioinformatics Online,2012,8(7):105-117.
[3] Shah P,Powell A L,Orlando R,et al.Proteomic analysis of ripening tomato fruit infected by Botrytis cinerea[J].Journal of Proteome Research,2012,11(4):2178-2192.
[4] Espino J J,Gutierrez-Sanchez G,Brito N,et al.The Botrytis cinerea early secretome[J].Proteomics,2010,10(16):3020-3034.
[5] Robles L M,Wampole J S,Christians M J,et al. Arabidopsis enhanced ethylene response 4 encodes an EIN3-interacting TFIID transcription factor required for proper ethylene response,including ERF1 induction[J].Journal of Experimental Botany,2007,58(10):2627-2639.
[6] Canonne J,Marino D,Jauneau A,et al.The Xanthomonas type Ⅲ effector XopD targets the Arabidopsis transcription factor MYB30 to suppress plant defense[J].Plant Cell,2011,23(9):3498-3511.
[7] Li L,Yu X,Thompson A,et al. Arabidopsis MYB30 is a direct target of BES1 and cooperates with BES1 to regulate brassinosteroid-induced gene expression[J].The Plant Journal,2009,58(2):275-286.
[8] Veronese P,Chen X,Bluhm B,et al.The BOS loci of Arabidopsis are required for resistance to Botrytis cinerea infection[J].The Plant Journal,2004,40(4):558-574.
[9] Denby K J,Kumar P,Kliebenstein D J.Identification of Botrytis cinerea susceptibility loci in Arabidopsis thaliana[J].The Plant Journal,2004,38(3):473-486.
[10] Veronese P,Nakagami H,Bluhm B,et al.The membrane-anchored BOTRYTIS-INDUCED KINASE1 plays distinct roles in Arabidopsis resistance to necrotrophic and biotrophic pathogens[J].Plant Cell,2006,18(1):257-273.
[11] Jihong X,Qiaoyu W,Congcong H,et al. T1N6-22 gene is required for biotic and abiotic stress responses in Arabidopsis[J].Russian Journal of Genetics,2012,48(12):1191-1198.
[12] Ziqiang L,Yan Z,Juan G,etal.Molecular and reverse genetic characterization of NUCLEOSOMEASSEMBLY PROTEIN1(NAP1)genes unravels their function in transcription and nucleotide excision repair in Arabidopsis thaliana[J].The Plant Journal,2009,59(59),27-38.
[13] Harald H O,Jennifer L A,Heather M W,et al.Organisation of the pantothenate(vitamin B5)biosynthesis pathway in higher plants[J].The Plant Journal,2004,37(1):61-72.
[14] He X J,Mu R L,Cao W H,et al.AtNAC2,a transcription factor downstream of ethylene and auxin signaling pathways,is involved in salt stress response and lateral root development[J].The Plant Journal,2005,44(6):903-916.
[15] Kerry A L,Jessica R F,Jeremy M E,et al.Peroxisomal metabolism of propionic acid and isobutyric acid in plants[J].The Journal of Biological Chemistry,2007,282(34):24980-24989.
[16] Jia Y L,MeAdams S A,Bryan G T,et al.Direct interaction of resistance gene and avirulence gene products confers rice blast resistance[J].The EMBO Journal,2000,19(15):4004-4014.
[17] Kim J H,Nguyen N H,Jeong C Y,et al.Loss of the R2R3 MYB, AtMyb73,causes hyper-induction of the SOS1 and SOS3 genes in response to high salinity in Arabidopsi s[J].Journal of Plant Physiology,2013,170(16):1461-1465.
[18] 樊锦涛,贾娇,蒋琛茜,等.拟南芥转录因子AtMYB73转录活性区域分析及互作蛋白的筛选[J].中国农业科学,2014,47(23):4754-4762.
[19] 王加峰,刘浩,王慧,等.水稻NBS-LRR类抗稻瘟病蛋白Pik-h的互作蛋白筛选[J].中国农业科学,2016,49(3):482-490.
[20] 孙海桃,徐兆师,侯建华,等.小麦TaDREB6转录因子互作蛋白的筛选[J].中国农业科学,2011,44(22):4740-4747.
[21] Vander E S,Verhagen B W,Van D R,et al.MYB72 is required in early signaling steps of rhizobacteria-induced systemic resistance in Arabidopsis[J].Plant Physiology,2008,146(3):1293-1304.
[22] García M J,Suárez V,Romera F J,et al.A new model involving ethylene,nitric oxide and Fe to explain the regulation of Fe-acquisition genes in Strategy I plants[J].Plant Physiology and Biochemistry,2011,49(5):537-544.
[23] Wu Q,Wang X Z,Tang Y Y,et al.Molecular cloning,genomic organization and functional analysis of the ribosomal protein L4/L1(RPL4)gene from from Arachishypogaea[J].Canadian Journal of Plant Science,2014,94(1):85-97.
[24] 王立丰,王纪坤,安锋,等.巴西橡胶树核糖体蛋白HbRPL14基因逆境响应机制[J].西南林业大学学报,2016,36(2):67-77.
[25] 曹广英,吴琪,唐月异,等.花生核糖体蛋白L29-1(RPL29-1)基因克隆表达分析及载体构建[J].分子植物育种,2016,14(7):1730-1736.
[26] Kim K Y,Park S W,Chung Y S,et al.Molecular cloning of low-temperature-inducible ribosomal proteins from soybean[J].Gene Note,2004:1153-1155.

Please choose a citation manager

Content to export