Envelope Protein of Goose Tembusu virus Induces Immune Response in Mice

doi:10.7668/hbnxb.2017.02.008

Abstract

Abstract: In order to research the immunogenicity of Goose tembusu virus (GTV) envelope protein (E protein),GTV E protein was expressed in Escherichia coli, and administered to BALB/c mice via subcutaneous injection to study the immune responses. After immunization by recombinant E protein, the titres of neutralizing antibody in immunized BALB/c mice were all above 1:51 200. Cytokine analysis of the serum of mice immunized with recombinant E protein revealed antigen-specific IL-2, TNF-α and IFN-γ responses. IL-2 and TNF-α in serum from immunized mice were extremely significantly higher than control mice (P<0.01) and IFN-γ was significantly higher than that of control mice (P<0.05).Additionally, the proliferation of lymphocytes from spleen of mice immunized with recombinant E protein was stimulated. The results indicated that recombinant GTV E protein could effectively stimulate humoral and lymphocyte immune responses in mice. Moreover, when mice were challenged with GTV, by using Real-time RT-PCR, the loads of virus in tissues from mice immunized with recombinant E protein were significantly decreased, revealing that recombinant E protein contributed to protection against GTV challenge. This study demonstrated that the E. coli-expressed recombinant GTV E protein could serve as a potential subunit vaccine candidate against GTV and provided a theoretical basis for the prevention and control of Tembusu virus.

Key words: Tembusu virus, Envelope protein, Immune response, Subunit vaccine

CLC Number:

S432.4+2

ZHAO Dongmin, HUANG Xinmei, HAN Kaikai, LIU Yuzhuo, YANG Jing, LIU Qingtao, BI Keran, LI Yin. Envelope Protein of Goose Tembusu virus Induces Immune Response in Mice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(2): 50-54. doi: 10.7668/hbnxb.2017.02.008.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2017.02.008

http://www.hbnxb.net/EN/Y2017/V32/I2/50

References

[1] Su J, Li S, Hu X, et al. Duck egg-drop syndrome caused by BYD virus, a new Tembusu-related flavivirus[J]. PLoS One, 2011, 6(3):e18106.
[2] Huang X, Han K, Zhao D, et al. Identification and molecular characterization of a novel Flavivirus isolated from geese in China[J]. Research in Veterinary Science, 2013, 94(3):774-780.
[3] Yan P, Zhao Y, Zhang X, et al. An infectious disease of ducks caused by a newly emerged Tembusu virus strain in mainland China[J]. Virology, 2011, 417(1):1-8.
[4] Niu H M, Huang X M, Han K K, et al. Development of double antibody sandwich ELISA for detection of duck or goose flavivirus[J]. Journal of Integrative Agriculture, 2013, 12(9):1638-1643.
[5] Zou G, Puig-Basagoiti F, Zhang B, et al. A single-amino acid substitution in West Nile virus 2K peptide between NS4A and NS4B confers resistance to lycorine, a flavivirus inhibitor[J]. Virology, 2009, 384(1):242-252.
[6] Bai X, Lü R, Liu C, et al. Molecular characterization of a duck Tembusu virus from China[J]. Virus Genes, 2013, 47(3):478-482.
[7] Lindenbach B, Rice C. Molecular biology of flaviviruses[J]. Advances in Virus Research, 2003, 59(7):23-61.
[8] Mukhopadhyay S, Kuhn R, Rossmann M. A structural perspective of the flavivirus life cycle[J]. Nature Reviews Microbiology, 2005, 3(1):13-22.
[9] Youn S, Cho H, Fremont D, et al. A short N-terminal peptide motif on flavivirus nonstructural protein NS1 modulates cellular targeting and immune recognition[J]. Journal of Virology, 2010, 84(18):9516-9532.
[10] Yu K, Sheng Z, Huang B, et al. Structural, antigenic, and evolutionary characterizations of the envelope protein of newly emerging Duck tembusu virus[J]. PLoS One, 2013, 8(8):e71319.
[11] Martínez C, Giulietti A, Talou J. Research advances in plant-made flavivirus antigens[J]. Biotechnology Advances, 2012, 30(6):1493-1505.
[12] 黄欣梅,李银,赵冬敏,等.鹅黄病毒囊膜蛋白的原核表达及抗原性分析[J].华北农学报, 2012, 27(5):33-37.
[13] Liu M, Chen S, Chen Y, et al. Adapted tembusu-like virus in chickens and geese in China[J]. Journal of Clinical Microbiology, 2012, 50(8):2807-2809.
[14] Tang Y, Diao Y, Yu C, et al. Characterization of a Tembusu virus isolated from naturally infected house sparrows (passer domesticus) in northern China[J]. Transboundary and Emerging Diseases, 2013, 60(2):152-158.
[15] Li S, Li X, Zhang L, et al. Duck tembusu virus exhibits neurovirulence in BALB/c mice[J]. Virology Journal, 2013, 10(1):260.
[16] Liu Z, Ji Y, Huang X, et al. An adapted duck Tembusu virus induces systemic infection and mediates antibody-dependent disease severity in mice[J]. Virus Research, 2013, 176(1/2):216-222.
[17] Tang Y, Gao X, Diao Y, et al. Tembusu virus in human, China[J]. Transboundary and Emerging Diseases, 2013, 60(3):193-196.
[18] Diamond M, Shrestha B, Marri A, et al. B cells and antibody play critical roles in the immediate defense of disseminated infection by West Nile encephalitis virus[J]. Journal of Virology, 2003, 77(4):2578-2586.
[19] Diamond M. Evasion of innate and adaptive immunity by flaviviruses[J]. Immunology and Cell Biology, 2003, 81(3):196-206.
[20] Monath T P. Treatment of yellow fever[J]. Antiviral Research, 2008, 78(1):116-124.

Please choose a citation manager

Content to export