ACTA AGRICULTURAE BOREALI-SINICA

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Immunological Analysis of the E2 Glycoprotein of Classical Swine Fever Virus in Rabbits

TIAN Hong, WU Jin-yan, YIN Shuang-hui, SHANG You-jun, ZHENG Hai-xue, LIU Xiang-tao   

  1. Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Sciences, Lanzhou 730046, China
  • Received:2009-10-28 Published:2009-12-31

Abstract: To investigate the immunogeneicity of a subunit vaccine of E2 glycoprotein of classical swine fever virus(CSFV). In this study, the rabbits were immunized with the homemade antigen (recombinant E2 protein), Tlymphocyte proliferation response, blocking ELISA and virus attacked test were used to detect the effect of the immunized responses in guinea pigs. The results indicated that a retroviral based vaccine carrying the E2 gene of CSF was able to elicit strong CSFV specific humoral immune responses in guinea pigs. These results encourages further work towards the development of a vaccine against CSFV infection.

Key words: Classical swine fever virus (CSFV), Subunit vaccine, E2 protein, Immunized test

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Cite this article

TIAN Hong, WU Jin-yan, YIN Shuang-hui, SHANG You-jun, ZHENG Hai-xue, LIU Xiang-tao. Immunological Analysis of the E2 Glycoprotein of Classical Swine Fever Virus in Rabbits[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S2.050.

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