Study on the Purifying of the Antagonistic Protein and the Isolation of Antagonistic Strain of Cotton Wilt

doi:10.7668/hbnxb.2009.S2.051

Abstract

Abstract: 108 Bacillus strain has been isolated from the roots of cotton, LC-105 strain has been chosen by the antagonistic effect to the cotton wilt and diameter of the antagonistic circle reach more than 20 mm. Antagonistic material has been separated from the metabolism of the strain. Antagonistic material purifying has been done by DEAE-Sephadex iron-exchange chromatography and Sephacryl-S100 gel chromatography. The antagonistic material is proved to be a kind of protein. Electrophoresis pure has been identified by the polyacrylamide gel electrophoresis method.

Key words: Cotton, Wilt, Antagonistic strain, Antagonistic material, Separating and purifying, Gel electrophoresis

CLC Number:

S435.62

ZHANG Shuang-feng, ZHANG Ai-min, ZHAO Gang-yong, ZHANG Ke-hong. Study on the Purifying of the Antagonistic Protein and the Isolation of Antagonistic Strain of Cotton Wilt[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S2.051.

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http://www.hbnxb.net/EN/Y2009/V24/IS2/229

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