Effect of Ure B Subunit Vaccine Immunization on Serum Proteins in Dairy Cows

doi:10.3969/j.issn.1000-7091.2012.05.037

Abstract

Abstract: This study was to investigate the changes of serum proteins in dairy cows immunized with Ure B subunit vaccine.Eight healthy cows were divided into 2 groups and immunized with 0.2 mg antigen protein or saline by intramuscular injection on both sides of the hip,once every two weeks,and total 4 times for immunization.Serum samples were collected on 56 d after immunization with Ure B subunit vaccine.Specific antibody in blood samples were determined by ELISA methods.Serum proteomic analysis were performed using two-dimensional gel electrophoresis(2-DE) coupled with MALDI-TOF-TOF mass spectrometer.Specific IgG were significantly higher after the cows immunized with Ure B subunit vaccine than control groups(P0.05),whereas IgM and IgA was not significant(P0.05).Proteomic analysis revealed 6 protein spots were differentially expressed,and 3 spots were successfully identified.Haptoglobin precursor and 3 unknown spots were up-regulated in cows immunized with Ure B subunit vaccine,while Kakapo was down-regulated.The current study showed that cows injection with Ure B subunit vaccine can result in the change of haptoglobin,Kakapo and other proteins,which provide the basis for further research of the physiology effects of Ure B subunit vaccine on dairy cows.

Key words: Ure B subunit vaccine, Dairy cow, Scrum Proteome

CLC Number:

S823

YUAN Ting-jie, WANG Jia-qi, YANG Yong-xin, BU Deng-pan, ZHANG Yu-tao, ZHAO Sheng-guo, ZHOU Ling-yun, LI Fa-di. Effect of Ure B Subunit Vaccine Immunization on Serum Proteins in Dairy Cows[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(5): 198-202. doi: 10.3969/j.issn.1000-7091.2012.05.037.

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