Effect of Artificial Insemination with Frozen-sexed Sperm on the Reproduction of Dairy Cow

doi:10.7668/hbnxb.2010.01.022

Abstract

Abstract: To evaluate the effect of artificial insemination with frozen-sexed sperm on the reproductive performance of dairy cow,and to further improve insemination strategies,frozen spermwassexed by flow cytometry. Then 3 947 health Holstein cow were artificially inseminated with frozen sperm unsexed or sexed. The results showed:①The pregnancy and calving rates were 48.91% and 97.97% for sexed-sperm AIs, 51.74% and 98.05% for the unsexed-sperm AIs(P>0.05).The proportion of female calves derived from sexed-sperm AIs was 92.01% compared with 51.13% for control AIs (P<0.01), the reproductive rate was 51.13% for sexed-sperm AIs and 34.97% for control AIs(P<0.01).② The pregnancy rate was 55.25% for primiparous cow and was obviously higher than that of multiparous cow (P<0.01), the calving rate was 98.31% for primiparous cow and was higher than that of multiparous cow(P<0.05), the reprodcutive rates was 51.84% for primiparous cow and 42.12% for multiparous cow, there were significant differences (P<0.01).③When a insemination was followed by a second one 10-11 hours later, and a single-doses sperm was used in each time, the pregnancy and reproductive rates were both the highest (P<0 01).Our results indicated that AI using sexed sperm under field conditions in commercial dairy herds without oestrus synchronization resulted in a significantly improvement of reprocuctive effect compared with AI using unsexed sperm.

Key words: Dairy cow, Sex control, Frozen sperm, Artificial insemination

CLC Number:

S814.3

MA Yi1,CHEN Xiao-qiang2,JIA Fu-de1,TIAN Yu-ze1,ZHANG Ju-zhong1,ZHU Fang-xian1,ZHAO Qing-bin1,WANG Lian-hai1. Effect of Artificial Insemination with Frozen-sexed Sperm on the Reproduction of Dairy Cow[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(1): 113-116. doi: 10.7668/hbnxb.2010.01.022.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2010.01.022

http://www.hbnxb.net/EN/Y2010/V25/I1/113

References

[1] Johnson L A,WelchG R,Rens W.The beltsville spermsexing technology:High-speed sperm sorting gives improved sperm output for in vitro fertilization and AI[J].J Anim Sci,1999,77 (Suppl.2):213-220.
[2] Seidel G E Jr,Schenk J L,Herickhoff L A,et al Insemination of heifers with sexed spermatozoa[J].Theriogenology,1999,52(8):1407-1420.
[3] Buchanan B R,Seidel G E Jr,McCue P M,et al.Insemination of mares with low numbers of either unsexed or sexed spermatozoa[J].Theriogenology,2000,53(6):1333-1344.
[4] Seidel G E Jr,Garner D L.Current status of sexing mammalian spermatozoa[J].Reproduction,2002,124(6):733-743.
[5] Tubman L M,Brink Z,Suh T K,et al.Characteristics of calves produced with sperm sexed by flow cytometry/cell sorting[J].J Anim Sci,2004,82(4):1029-1036.
[6] Maxwell,W M,Johnson L A.Physiology of spermatozoa at high dilution rates:the influence of seminal plasma[J].Theriogenology,1999,52(8):1353-1362.
[7] Morrell J M,Dresser D W.Offspring from insemination with mammalian sperm stained with Hoechst 33342,either with or without flow cytometry[J].Mutation Research,1989,224(2):177-189.
[8] Johnson L A,Welch G R,Keyvanfar K,et al.Gender preselection in humans? Flow cytometric separation of X and Y spermatozoa for the prevention of X-linked diseases[J].Human Reproduction,1993,8(10):1733-1739.
[9] Doyle S P,Seidel G E Jr,Schenk J L,et al.Artificial insemination of lactating Angus cows with sexed semen[M]//American Society of Animal Science Proceedings.Provo,Utah:American Society of Animal Science,1999:203-205.
[10] Fugger E F.Clinical experience with flow cytometric separation of human X-and Y-chromosome bearing sperm[J].Theriogenology,1999,52(8):1345-1440.
[11] Andersson M,Taponen J,Koskinen E,et al.Effect of insemination with dosesof 2 or 15 million frozen-thawed spermatozoa and semen deposition site on pregnancy rate in dairy cows[J].Theriogenology,2004,61(7-8):1583-1588.
[12] Den Daas J H,De Jong G,LansbergenL M,et al.The relationship between the number of spermatozoa inseminated and the reproductive efficiency of individual dairy bulls[J].J Dairy Sci,1998,81(6):1714-1723.
[13] Schenk J L,Suh T K,Seidel G E Jr.Embryo production from superovulated cattle following insemination of sexed sperm[J].Theriogenology,2006,65(2):299-307.
[14] Garcia Ispierto I,Lopez-Gatius F,Santolaria P,et al.Factors affecting the fertility of high producing dairy herds in northeastern Spain[J].Theriogenology,2007,67(3):632-638.
[15] Caraviello D Z,Weigel K A,Fricke P M,et al.Survey of management practices on reproductive performance of dairy cattle on large US commercial farms[J].J Dairy Sci,2006,89(12):4723-4735.

Please choose a citation manager

Content to export