Analysis of the Progeny from Wide Cross T.timopheevi and Avena fatua L.var.Portugal Using SRAP Technique

doi:10.7668/hbnxb.2010.01.021

Abstract

Abstract: The hybrid progenies from wide cross T.timopheevi×Avena fatua L.var.Portugal were analyzed using SRAP markers to prove their truth and understand their characterizations in the present study.Among 22 primer pairs,20 yielded the polymorphic product between the both parents,and the average polymorphic ratio was 73.81%.Four primer pairs (me4-em1,me3-em5,me⁴-em3 and me3-em3) produced the specific markers for either parent in the F₃ line,indicating that the F₃ line contained both parental homologues of some chromosomes,and were true intergeneric hybrid origin.The F₃ line lost some parental SRAP markers,and the markers lost from T.timopheevi were far less than those from the wild oat;meanwhile,some novel SRAP markers were generated.These DNA sequence changes might contribute to the rapid evolution of the newly formed allopolyploid and allow the divergent genomes to act in harmony.

Key words: T.timopheevi, Avena fatua L.var.Portugal, Wide cross, SRAP

CLC Number:

S512.1
Q812

GENG Guang-dong,ZHANG Su-qin,JIA Kai-jia,XU Ru-hong,REN Ming-jian,ZHANG Qing-qin. Analysis of the Progeny from Wide Cross T.timopheevi and Avena fatua L.var.Portugal Using SRAP Technique[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(1): 110-112. doi: 10.7668/hbnxb.2010.01.021.

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