Construction of HPV45-L1 and HPV58-L1 Plant Expression Vectors and Identification of Transgenic Rice Plants

doi:10.7668/hbnxb.2017.02.009

Abstract

Abstract: The rice seeds were used as the cell bioreactor to construct the recombinant plant expression vector containing HPV45-L1 and HPV58-L1 genes to provide a new efficient and inexpensive method for HPV45-L1 and HPV58-L1 protein expression.The HPV45-L1 and HPV58-L1 coding sequence (ORF)was amplified by PCR and then subcloned into middle vector pMP3 and plant expression vector pCAMBIA-1300 to construct the rice endosperm-specific expression vector pCAMBIA-1300-pMP3-HPV45-L1 and pCAMBIA-1300-pMP3-HPV58-L1.Subsequently,the transgenic rice plants containing HPV-L1 ORF gene were obtained by agrobacterium-mediated genetic transformation of rice callus.The PCR test showed that the HPV45-L1 and HPV58-L1 genes had integrated into the rice genome,suggesting the successful construction of rice endosperm specific expression vectors.

Key words: Human papilloma virus, Transgenic rice, The rice specific endosperm-promoter, Agrobacterium tumefaciens

CLC Number:

Q78
S511.03

WANG Jucai, LIU Yunchao, CHEN Yumei, MENG Fengli, WANG Aiping, ZHANG Erqin, XU Qianru, DENG Ruiguang, ZHANG Gaiping. Construction of HPV45-L1 and HPV58-L1 Plant Expression Vectors and Identification of Transgenic Rice Plants[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(2): 55-60. doi: 10.7668/hbnxb.2017.02.009.

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