Abstract: To elucidate the biological structure basis of transgenic rice plants expressing maize PEPC gene with high photosynthesis efficiency, PEPC transgenic rice and its wild type with at 3 leaves seedling stage were treated under 1000 μmol/(m²·s) light intensity for 100 min, respectively, and those under 200 μmol/(m²·s) light as the control. The structure of mesophyll cells, bundle sheath and thylakoids of the second blade were observed with a transmission electron microscope. The results showed that compared with the control, the choloplasts of transgenic rice were arranged denser, the stacking of thylakoids was in a much order condition, the ordered lamella was more thicker and arranged denser, the structure of thylakoids became more completed and the mitochondia among the chloroplast increased after strong light treatment. But, the lamella of thylakoids in WT was looser and the structure of them were confusion even disorganization under strong light, additionally, the amount of amyloplastsin in the stroma of WT under strong light had an obviously increase. It indicated that the photosynthesis organs of the blades of PEPC transgenic rice under strong light had a steady micro-structure, which could be the structure basis for higher light utilization ability, therefore increasing photosynthesis ability.

Key words: Transgenic rice of PEPC, Thylakoids, Mesophyll cells, Micro-structure

CLC Number: 

• S513.01