A Simplified Method for Observing Stomata by Shaving off Mesophyll Cells to Obtain Epidermis from Leaf and Its Application

doi:10.7668/hbnxb.2008.S1.019

Abstract

Abstract: In order to facilitate the process of plant stomatal development and physiology study, we report a simplified method for observing stomata by using transparent tape to shave off mesophyll cells and obtain epidermis from leaf.Compared with other methods: by tearing or adhering, this method is simple, efficient and wide applied. It has a good application on soybeans, wheat, corn and other crops. It is suitable for studying different developmental stages, distribution,dynamics and morphological studies of stomata.

Key words: Transparent tape, Leaf, Epidermis, Shaving off mesophyll cells, Stomata

CLC Number:

Q944

DUAN Yun-feng, WANG You-ning, LI Xia,. A Simplified Method for Observing Stomata by Shaving off Mesophyll Cells to Obtain Epidermis from Leaf and Its Application[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S1): 73-76. doi: 10.7668/hbnxb.2008.S1.019.

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