Biological Characteristics and Sensitivity to Fungicides of Bipolaris sorokiniana Causing Maize Leaf Spot

doi:10.7668/hbnxb.20190188

Abstract

Abstract: Bipolaris sorokiniana is a causal agent of leaf spot disease in maize. The biological characteristics of B. sorokiniana including mycelial growth, sporulation and conidial germination at different temperature, pH and carbon/nitrogen sources were determined, and the sensitivity of this pathogen to 8 commonly used fungicides was evaluated. The results showed that the mycelial growth and conidial germination rate were the highest at 28 ℃, which were 73.00 mm and 96.91%, respectively. The optimum temperature of sporulation was 25 ℃, and the amount of spore was 15.13×10³/cm². The mycelium grew fastest on the media with pH 7 and 8, which were 65.25，65.13 mm, respectively, and the conidial germination rates were 88.75% and 85.00% at the pH 7 and 8, respectively. The highest sporulation was observed on the medium of pH 7 with 48.44×10³/cm². The mycelial growth on the media with starch and sucrose as carbon sources were 53.00, 50.17 mm, respectively. The highest sporulation on the medium with galactose as carbon source was 1.93×10⁴/cm², followed by the medium with starch, and the amount of spore was 0.42×10⁴/cm². The conidial germination rates on the medium with starch, galactose and sucrose as carbon sources were 76.03%, 74.52% and 72.26%, respectively. The growth of mycelium was the fastest on the medium with sodium nitrate as the nitrogen source, which was 36.44 mm. The highest sporulation was 5.59×10³/cm² on the medium with proline as the nitrogen source. And the conidial germination rates on the medium with proline and sodium nitrate as nitrogen sources were 49.38% and 45.00%, respectively. The results of sensitivity to tested fungicide indicated that the inhibitory activity of tebuconazole and fludioxonil to mycelial growth was the highest with the same EC₅₀of 0.07 mg/L and that of fludioxonil to conidial germination was the best with EC₅₀ of 2.80 mg/L. The results of this study will provide a theoretical basis for understanding the occurrence and prevalence of B. sorokiniana leaf spot and formulating effective control measures.

Key words: Maize leaf spot, Bipolaris sorokiniana, Biological characteristic, Funigicide, Inhibitory activity

CLC Number:

S435.131

GUO Ning, HU Qingyu, LIU Yueyang, SHI Jie, ZHANG Haijian, LIU Shusen. Biological Characteristics and Sensitivity to Fungicides of Bipolaris sorokiniana Causing Maize Leaf Spot[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(S1): 289-295. doi: 10.7668/hbnxb.20190188.

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