The Study on Biological Characteristics of GFP Labeled Potato Verticillium dahliae

doi:10.7668/hbnxb.2016.06.014

Abstract

Abstract: In order to unravel the infection mechanism of V.dahliae to potato,a binary construct contained GFP gene was transferred via Agrobacterium tumefaciens-mediated system into V.dahliae isolate VD012.47 positive transformants were obtained by hygromycin B selection and molecular verification.Eight tansformants were randomly selected to characterize the colony morphology,growth rate,conidia production,toxin production and pathogenicity.The results showed that three transformants could produce more microsclerotia compared with the wild type strain;whereas,one transforment produced less microsclerotia.The significant difference on the growth rate of eight transformants. The conidia production ability of the transformants were decreased to certain degree.except two among them were not remarkable overall.The average germination rate of all the tested transformants was lower than that of the wild strain after 8 h germination.Regarding to the toxin production,seven transformants showed increased tendency,while only one decreased compared with the wild type.The pathogencity results showed that,compared with wild strain,only one transformant showed increased tendency,the left two strains showed decreased pattern.

Key words: Potato wilt, Verticillium dahliae, GFP labelled transformants, Biological characteristics

CLC Number:

S435.32

WANG Kai, DONG Baozhu, ZHANG Gui, ZHANG Jian, ZHANG Yuanyuan, ZHOU Hongyou, ZHAO Jun. The Study on Biological Characteristics of GFP Labeled Potato Verticillium dahliae[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(6): 88-93. doi: 10.7668/hbnxb.2016.06.014.

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http://www.hbnxb.net/EN/Y2016/V31/I6/88

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