Abstract: The present study aimed to establish ear skin fibroblasts(cGSF)from a cloned Huanghuai white goat and explore the biological characteristics of the established cGSF cell line.The results were as follows:Both explants adherence culture(A method)and trypsin digestion(B method)could result in the successful growth of primary fibroblasts.However,after 24 h in culture,adherence rate of cells harvested from A method was higher than that of B method.The amount of harvested cells using A method was also greater than using B method.As for the growing characteristics,the growth curve of cGSF cells was typical "S" type.For the time spent on reaching to the exponential growth phase(Day 3 Vs.Day 4)and the platform phase(Day 6 Vs.Day 8),cGSF was earlier than control(non-cloned goat fibroblast cells). We also found that rate of chromosomal abnormalities in cells from cloned goats was higher than those from non-cloned.In order to test feasibility of transfection using cGSF,pEGFP-N1 plasmid was subjected to be transfected with cultured cGSF,and we found that cGSF cells were more easier to form clonies after G418 screen.Therefore,if cells from cloned animals are employed in transgenic research,appropriate readjustment in cell isolation, in vitro culture,transfection and positive cell screen and enrichment should be done accordingly.In all,cells in vitro derived form cloned goat show differences in biological characteristics from cell lines of non-cloned goat.

Key words: Somatic cloning, Goat, Fibroblast cells, Biological characteristics

CLC Number: 

• Q78

• S826