Abstract: Intron 3 of the ovine GH gene mutation of Gansu alpine fine wool sheep growth traits may have effects and the gene markers to provide reference of Gansu alpine fine wool sheep growth candidate.Gansu alpine merino and Texel merino,Bond merino,Australia merino crossbred sheep GH gene intron 3 polymorphism was detected by PCR-SSCP technique,analysis of GH gene mutation in intron 3 of lamb birth weight,three-month weight,weaning weight and daily gain influence.The results showed that the first mutation between the Gansu alpine merino and crossbred lamb was GH gene intron 3 of 98 bp a C>T mutation,and this mutation gives AA,AB and BB three genotypes,Gansu alpine merino and crossbred sheep in the control group that the PIC values were between 0.25-0.50,was moderate polymorphism.The dominant genotype of which was BB.Alleles associated with the presence or absence of body weight and daily gain analysis showed the presence of allele B of Gansu alpine merino 3-month weight,weaning weight and daily gain were significantly higher than the individual's lack of allele B(P<0.05),deletion allele a special Texel Gansu alpine merino in birth weight,weaning weight and average daily gain was significantly higher than that of the existence of areas a allele individuals(P<0.05).Bond Gansu alpine merino of allele B 3-month weight,weaning weight,average daily gain were significantly higher than the B allele missing individuals.Correlation analysis showed that different genotypes of Gansu alpine merino correlation between birth weight was not significant,BB type Gansu alpine merino of 3-month weight and weaning weight were significantly higher than AA genotype(P<0.05),average daily gain of AA and BB genotypes Gansu alpine merino were also significantly correlated(P<0.05).In the control group,Bond Gansu alpine merino BB type of birth weight,weaning weight and average daily gain were significantly higher than those of AA genotype(P<0.01).Bond Gansu alpine merino,Australian merino,Gansu alpine merino different genotypes with body weight and average daily gain were no correlation(P>0.05).The results showed that the intron 3 of GH gene could be used as a marker of growth traits of Gansu Alpine Merino candidate gene.

Key words: Gansu alpine merino, GH gene intron 3, Polymorphism, Growth traits

CLC Number: 

• Q78

• S826