Research on Differential Expressed Genes in Maize under Low-zinc and Zinc-sufficiency Conditions

doi:10.7668/hbnxb.2007.04.022

Abstract

Abstract: Suppression subtractive hybridization (SSH) was performed to isolate the differentially expressed cDNA fragments under low-zinc and zinc-sufficiency conditions. In the forward subtracted library, the cDNA of maize seedlings treated with low-zinc was used as tester and the cDNA of maize seedlings treated with zinc-sufficiency was used as driver. In the reverse subtracted library, the cDNA of maize seedlings treated with zinc-sufficiency was used as tester and the cDNA of maize seedlings treated with low-zinc was used as driver. The cDNA fragments differentially expressed under low-zinc and zinc-sufficiency conditions were collected in the forward and reverse subtracted library, respectively. Thirty clones were randomly picked to sequence in each library. The sequencing results shows: the function of isolated genes in the forward subtracted library mostly relates to disease resistance/defense, signal transduction, cell growth/division and so on. While the function of isolated genes in the reverse subtracted library mostly relates to cell/tissue generation, metabolism and so on.

Key words: Suppression subtractive hybridization, Maize, Low-zinc

CLC Number:

Q786

LU Meng-meng, WANG Jing-an. Research on Differential Expressed Genes in Maize under Low-zinc and Zinc-sufficiency Conditions[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 94-98. doi: 10.7668/hbnxb.2007.04.022.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2007.04.022

http://www.hbnxb.net/EN/Y2007/V22/I4/94

References

[1] Sillanp(aa) M. Micronutrients and the Nutrient Status of Soils: A global Study[M]//FAO, Soils Bulletin Rome: FAO, 1982.
[2] 徐晓燕, 杨肖娥, 杨玉爱. 锌在植物中的形态及生理作用机理研究进展[J]. 广东微量元素科学, 1999, 6(11): 1-4.
[3] 孙桂芳, 杨光穗. 土壤-植物系统中锌的研究进展[J]. 华南热带农业大学学报, 2002, 8(2): 22-30.
[4] Gitan R S, Luo H, Rodgers J, et al. Zinc-induced inactivation of the yeast ZRT1 zinc transporter occurs through endocytosis and vacuolar degradation[J]. Biol Chem, 1998, 273: 28617-28624.
[5] Gitan R S, Shababi M, Kramer M, et al. A cytosolic domain of the yeast Zrtl zinc trasporter is required for its post-translational inactivation in response to zinc and cadmium[J]. Biol Chem, 2003, 278: 39558-39564.
[6] Singh S P, Wetermann D T. A single dominant gene controlling resistance to soil zinc deficiency in common bean[J]. Crop Sci, 2002, 31: 23-29.
[7] Forster S M, Moraghan J T, Grafton K F. Inheritance of seedZn accumulation in navy bean[R]. Annu Rpt Bean Improv Coop, 2002, 45: 30-31.
[8] Diachenko L, LauY F, Campbell A P, et al. Suppression subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries[J]. Pro Natl Acad Sci, 1996, 93: 6025-6030.
[9] Chen X, Yuan H, Chen R, et al. Isolation and characterization of triacontanol-regulated genes in rice (Oryza Sativa L. ): possible role of triacontanol as a plant growth stimulator[J]. Plant Cell Physiol, 2002, 43(8): 869-876.
[10] 骆蒙, 孔秀英, 刘越, 等. 小麦抗病基因表达谱中的文库构建与筛选方法研究[J]. 遗传学报, 2002, 29(9): 814-819.
[11] 卢圣栋. 现代分子生物学实验技术[M]. 北京: 中国协和医科大学出版社, 2001.
[12] Bevan M, Bancroft I, Bent E, et al. Analysis of 1. 9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana[J]. Nature, 1998, 391: 485-488.
[13] Von Tein O D, Thies W G, Hofman M A. A high throughput screening for rarely transcribed differentially expressed genes[J]. Nucleic Acids Research, 1997, 25(13): 2598-2602.
[14] 王景安, 张福锁. 一定的低锌比缺锌对玉米危害更大[J]. 自然科学进展, 2002, 12(2): 205-207.
[15] 金谷雷, 汪旭升, 朱军. 水稻14-3-3蛋白家族的生物信息学分析[J]. 遗传学报, 2005, 32(7): 726-732.
[16] 王晓文, 范文红, 范明. Copines蛋白家族的研究进展[J]. 国外医学生理、病理科学与临床分册, 2005, 25(2): 153-155.
[17] 宋水山, 杨文香, 李亚宁, 等. 植物G蛋白与植物防卫反应[J]. 中国生物工程杂志, 2006, 26(3): 73-77.
[18] 李春秀, 齐力旺, 王建华, 等. 植物纤维素合成酶基因和纤维素的生物合成[J]. 生物技术通报, 2005, 4: 5-11.
[19] 王立德, 廖红, 王秀荣, 等. 植物根毛的发生、发育及养分吸收[J]. 植物学通报, 2004, 21(6): 649-659.
[20] 吴顺, 萧浪涛, 鲁旭东. 植物的生长素结合蛋白[J]. 植物生理学通讯, 2004, 40(2): 241-245.

Please choose a citation manager

Content to export