Increasing of Maize Amylose Content by Sense RNA Interference

doi:10.7668/hbnxb.2010.04.020

Abstract

Abstract: In order to explore the effect of sense RNAi (RNA interference) on increase the amylose content of maize (Zea mays) ，two sense-RNAi vectors pBAC506 and pBAC508 with the sense sbeⅡb and selection gene epsps or bar were constructed and transformed to maize callus induced from immature embryos of the elite inbred lines by biolistic PDS1000 /He. Forty-four T0 transformed plantlets were regenerated，and thirty plants were positive by analysis of PCR and PCR-Southern hybridization. Southern-blotting results of 9 strong PCR-positive plants showed that the RNAi constructs in 7 plants were successful integrated into their genomes，4 with one copy of transgene，2 with two copies and 1 with three copies. The amylose content of 2 T2 transgenic lines were 23. 22% and 24. 60%，respectively. The results suggested that a big transgenic population was necessary for screening the transgenic plants with multi-copy transgene and high amylose content.

Key words: Maize, Starch branching enzyme, Sense-RNAi, Amylose

CLC Number:

S336

ZHANG Gui-tang, LU Dong-chang-cheng, SUN Chong-xia, LIANG Rong-qi, YANG Feng-ping, ZHANG Xiao-dong. Increasing of Maize Amylose Content by Sense RNA Interference[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 92-96. doi: 10.7668/hbnxb.2010.04.020.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2010.04.020

http://www.hbnxb.net/EN/Y2010/V25/I4/92

References

[1] 王振华,亢伟民,张新.高淀粉玉米及其开发利用[J].玉米科学,2002,10(3):90-92.

[2] 张文青.抗性淀粉--功能性食物成分[J].国外医学卫生学分册,2005,2(4):232-235.

[3] 张志英,沈建福.抗性淀粉最新研究进展[J].粮油食品科技,2005,13(4):29-31.

[4] Visser R G,FJacobsen E.Towards modifying plants foraltered starch content and composition[J].Trends Biotechnol,1993,11:63-68.

[5] Smith A M,Denyer K,Martin C.The synthesis of thestarch granule[J].Annu Rev Plant Physiol Plant Mol Biol,1997,48:67-87.

[6] Fergason V.High amylose and waxy corns[C]//Hallauer A R.Specialty corn.Boca Rotan:1994,CRC Press.

[7] 陈艳萍,袁建华,颜伟,等.高直链淀粉玉米研究进展[J].南京农专学报,2002,18(3):32-40.

[8] Napoli C,Lemicux C,Jorgensen R.Introduction of a chalcone synthasa gene into Petunia results in reversibe co-suppression of homologous gene in trans[J].Plant Cell,1990,2:279.

[9] Van der Krol A P,Mur L A,Beld M,et al.Flavonoid genes in petunia:Addition of a limited number of gene copies may lead to a suppression of gene expression[J].Plant Cell,1990,2:291-299.

[10] Jorgensen R A,Ma C L,Doetsch N.Functional genomics by sense-RNAi:a forward genetic approach for cell-type-targeted mutagenesis[C].Plant Genomics in China Ⅵ,2005:1.

[11] Que Q,Wang H Y,English J J,et al,The frequency and degree of cosuppression by sense chalcone synthase transgenes are dependent on transgene promoter strength and are reduced by premature nonsense cordons in the transgene coding cequence[J].Plant Cell,1997,9(8):1357-1368.

[12] ZHANG Xiaodong,LIANG Rongqi,CHEN xuqing,et al.Transgene inheritance and quality improvement by expressing novel HMW glutenin subunit (HMW-GS)genes in winter wheat[J].Chinese Science Bulletin(科学通报英文版),2003,48(8):771-776.

[13] Pesole G,Liuni S,Grillo G,et al.UTRdb and UTRsite:specialize databases of sequences and functional elements of 5′ and 3′ untranslated regions of eukaryotic mRNAs[J].Nucleic Acids Research,2002,30:335-340.

[14] 王镜岩,朱圣庚,徐长法.生物化学[M].3版.北京:高等教育出版社,2002.

[15] Ohnishi Y,Tokunaga K,Hohjoh H.Influence of assembly of siRNA elements into RNA-induced silencing complex by fork-siRNA duplex carrying nucleotide mismatches at the 3′-or 5′-end of the sense-stranded siRNA element[J].Biochemical and Biophysical Research Communications,2005,329:516-521.

[16] 徐军望,李旭刚,朱祯.基因工程改良淀粉品质[J].生物技术通报,2000(1):11-19.

[17] 柴晓杰,王丕武,关淑艳,等.应用RNA干扰技术降低玉米支链淀粉含量[J].植物生理与分子生物学学报,2005,31(6):625-630.

[18] 郭新梅,张晓东,梁荣奇,等.利用RNAi技术提高玉米直链淀粉含量[J].农业生物技术学报,2008,16(4):658-661.

Please choose a citation manager

Content to export