Synthesis of Cimaterol Artificial Antigen and Development of Its Polyclonal Antiserum

doi:10.7668/hbnxb.2010.04.021

Abstract

Abstract: The goal of this study was to synthesize artificial antigen of cimaterol and obtain its mice polyclonal antiserum. Artificial antigen BSA-CIM and OVA-CIM were synthesized using diazotization by linking carrier proteins BSA and OVA to CIM. The antigen BSA-CIM and OVA-CIM was identified by ultraviolet scanning，SDS-PAGE. The results showed that the hapten CIM was successfully linked to carrier proteins. The animal immunization results showed that six BALB / c mice indirect ELISA titer against CIM were above 1 × 10^－3 and the IC₅₀ of No. 4 mice was 86. 9 ng /mL and the high-titer，sensitivity CIM polyclonal antiserum has been generated. The artificial antigen of CIM and its mice polyclonal antiserum laid a foundation base for product the monoclonal antibodies and rapid test reagent against CIM.

Key words: Cimaterol, Artificial antigen, Synthesis, Polyclonal antiserum

CLC Number:

S82.2

ZHI Ai-min, LIU Qing-tang, LI Qing-mei, YANG Su-zhen, HU Xiao-fei, CHAI Shu-jun, DENG Rui-guang, ZHANG Gai-ping. Synthesis of Cimaterol Artificial Antigen and Development of Its Polyclonal Antiserum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 97-101. doi: 10.7668/hbnxb.2010.04.021.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2010.04.021

http://www.hbnxb.net/EN/Y2010/V25/I4/97

References

[1] Jones R W,Easter R A,McKeith F K,et al.Effect of the beta-adrenergic agonist cimaterol (CL 263,780) on the growth and carcass characteristics of finishing swine[J].J Anim Sci,1985,61(4):905-913.

[2] Hill M A,Dalrymple R H.Evaluation of feet and skeletons of limbs from pigs treated with a repartitioning agent,cimaterol[J].Can J Vet Res,1987,51(2):217-223.

[3] Kim Y S,Lee Y B,Dalrymple R H.Effect of the repartitioning agent cimaterol on growth,carcass and skeletal muscle characteristics in lambs[J].J Anim Sci,1987,65(5):1392-1399.

[4] Sainz R D,Wolff J E.Effects of the beta-agonist,cimaterol,on growth,body composition and energy expenditure in rats[J].Br J Nutr,1988,60(1):85-90.

[5] Merkley J W,Cartwright A L.Adipose tissue deposition and cellularity in cimaterol-treated female broilers[J].Poult Sci,1989,68(6):762-770.

[6] Gwartney B L,Calkins C R,Jones S J.The effect of cimaterol and its withdrawal on carcass composition and meat tenderness of broiler chickens[J].J Anim Sci,1991,69(4):1551-1558.

[7] Fiems L O,Boucque C V,Cottyn B G.Effect of duration of a beta-agonist treatment on growth,feed intake and carcass characteristics in finishing bulls[J].Arch Tierernahr,1993,45(2):101-109.

[8] 金邦荃,周蔚,陈剑慧,等.塞曼特罗(cimaterol)对肉鸭生长后期肌肉增长和体脂代谢的影响[J].江苏农业学报,1998,14(2):118-122.

[9] Schiavone A,Tarantola M,Perona G,et al.Effect of dietary clenbuterol and cimaterol on muscle composition,beta-adrenergic and androgen receptor concentrations in broiler chickens[J].J Anim Physiol Anim Nutr (Berl),2004,88(3-4):94-100.

[10] 农业部畜牧兽医局.农业部发布动物性食品中兽药最高残留限量[J].中国兽药杂志,2003,37(4):15-20.

[11] Degroodt J M,Wyhowski de Bukanski B,De Groof J,et al.Cimaterol and clenbuterol residue analysis by HPLC-HPTLC in liver[J].Z Lebensm Unters Forsch,1991,192(5):430-432.

[12] Courtheyn D,Desaever C,Verhe R.High-performance liquid chromatographic determination of clenbuterol and cimaterol using post-column derivatization[J].J Chromatogr,1991,564(2):537-549.

[13] Fesser A C,Dickson L C,MacNeil J D,et al.Determination of beta-agonists in liver and retina by liquid chromatography-tandem mass spectrometry[J].J AOAC Int,2005,88(1):61-69.

[14] Van Vyncht G,Preece S,Gaspar P,et al.Gas and liquid chromatography coupled to tandem mass spectrometry for the multiresidue analysis of beta-agonists in biological matrices[J].J Chromatogr A,1996,750(1-2):43-49.

[15] Sun L,Zhang L,Zhu Y,et al.Simultaneous determination of nine beta-agonist residues in animal derived foods by ultra performance liquid chromatography-tandem mass spectrometry[J].Se Pu,2008,26(6):709-713.

[16] Wu P,Chen H,Wang Q,et al.Multi-residue analysis of 10 beta2-agonists in animal tissues using gas chromatography-mass spectrometry[J].Se Pu,2008,26(1):39-42.

[17] Duan J,Chen H,Chen Y,et al.Simultaneous determination of cimaterol,clenbuterol and salbutamol in feeds by capillary zone electrophoresis[J].Se Pu,2005,23(3):261-263.

[18] 段建平,陈红青,陈颖,等.高效毛细管电泳法同时测定西马特罗、盐酸克伦特罗、沙丁胺醇[J].广西师范大学学报:自然科学版,2003,1(21):250-251.

[19] 赵银丽,滕蔓,邓瑞广,等.恩诺沙星单克隆抗体杂交瘤细胞株的建立及其免疫学特性[J].河南农业科学,2008,10:121-124.

[20] 王选年,杨艳艳,邢广旭,等.盐酸克伦特罗单抗快速检测试剂盒的研制[J].中国兽医学报,2004,24(1):75-78.

[21] 刘宣兵,张改平,王斌,等.磺胺甲噁唑残留检测阻断ELISA试剂盒的研制[J].华北农学报,2009,24 (2):214-217.

[22] 张改平,职爱民,邓瑞广,等.兽药残留的免疫学快速检测技术概述[J].河南农业科学,2009(9):193-196.

[23] 范国英,王建华,王自良,等.链霉素残留快速检测阻断ELISA试剂盒的研制及其性能测定[J].中国兽医杂志,2008,44(1):82-84.

[24] Sambrook J,Fritsch E,Maniatis T.Molecular cloning:a laboratory manual[M].NY:Cold Spring Harbor Laboratory Press,1989.

[25] Tijssen P.Practice and theory of enzyme immunoassay[M].Amsterdam:Elsever,1985.

[26] 李林生.戊二醛与蛋白质反应的特点[J].中国皮革,1997(5):12-14.

[27] Wie S I,Hammock B D.Comparison of coating and immunizing antigen structure on the sensitivity and specificity of immunoassays for benzoylphenyl urea insecticides[J].Journal of Agricultural and Food Chemistry,1984,32(6):1294-1301.

Please choose a citation manager

Content to export