RFLP Analysis of Ribosomal DNA Internal Transcribed Spacers on Arca subcrenata,A.inflata and A.granosa

doi:10.7668/hbnxb.2010.04.019

Abstract

Abstract: In this paper，RFLP analysis was conducted in ribosomal DNA internal transcribed spacers (ITS) of Arca subcrenata，Arca inflata and Arca (anadara) granosa. The results showed that A. granosaa and A. subcrenata could be differentiated using restrictive enzymes of HaeⅢ，HhaⅠ，HpaⅡ，RsaⅠ，Sse9Ⅰand TaqⅠ，as well as A. granosaa and A. inflata. No specific RFLP marker was observed between A. subcrenata and A. inflata. Pnet value between A. subcrenata and A. inflata was zero，while Pnet values between A. granosa and the other two species were both 0.079 7. It was observed that there were 5 composite haplotypes in A. granosa and the nucleotide diversity index (π value) was 0.000 8，while one haplotype was observed in A. subcrenata and A. inflata and π values were both zero in these two species.

Key words: Arca subcrenata, Arca inflata, Arca (anadara) granosa, ITS, RFLP

CLC Number:

GUO Yong-jun, DAI Wei, WANG Xiao-mei, ZHOU Xiao-du. RFLP Analysis of Ribosomal DNA Internal Transcribed Spacers on Arca subcrenata,A.inflata and A.granosa[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 88-91. doi: 10.7668/hbnxb.2010.04.019.

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