Cloning of Maize ZmPT6 Gene and Construction of RNAi Expression Vector

doi:10.7668/hbnxb.2009.05.003

Abstract

Abstract: The arbuscular mycorrhizal symbiotic phosphate transporter gene ZmPT6 was cloned from Zea mays Zhengdan 958 genomic DNA and RNA by PCR method.Sequences analysis showed that ZmPT6 fulllength cDNA 1665bp,and contain a intron of 97bp.ZmPT6 belong to member of Pht1 family,encoded 554 amino acid,and consist of 12 putative hydrophobic transmembrane domains.Three plant transformation vectors,sense vector p2300-35S-ZmPT6,antisense p2300-35S-ZmPT6 r,and RNAi vector p2300-35S-ZmPT6 RNAi were constructed and so as to provide basis of further genetic transformation and function of ZmPT6 gene.

Key words: ZmPT6 gene, Sense expression vector, Antisense expression vector, RNAi vector

CLC Number:

Q785

ZHU Xian-can, SONG Feng-bin. Cloning of Maize ZmPT6 Gene and Construction of RNAi Expression Vector[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(5): 11-15. doi: 10.7668/hbnxb.2009.05.003.

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