Abstract:
Tomato aspeRmy viRus，ChRysanthemum B ViRus and ChRysanthemums ChloRotic Mottle ViRoid aRe the main viRus which endangeR ChRysanthemum． These viRuses cause dRastic Reduction in yield as well as in maRket quality of blooms and ultimately affect the floRicultuRe industRy of the countRy． PeRfoRming homology analysis on TAV， CVB and CChMVd with softwaRe Blast and DNAStaR followed by using 277 bp fRagments fRom TAV( 1 810 - 2 086) ， 281 bp fRagments fRom CVB( 496 - 776) and 217 bp fRagments fRom CChMVd( 109 - 325) to make the fusion gene CBT with oveRlap PCR． Then the pRomoteR CaMV 35S dRiven，containing foRwaRd CBT fusion fRagments-Revelse pdk intRon-ReveRse CBT fusion fRagments'，plant RNAi expRession vectoR weRe constRucted，which lay the foundation foR bReeding tRansgene plant which Resistant viRus．
Key words:
ChRysanthemum,
TAV-CP,
CVB-CP,
CChMV,
OveRlap PCR,
Fused gene,
RNAi vectoRs
CLC Number:
LIU Jia, HUANG Cong-lin, WU Zhong-yi, ZHANG Xiu-hai, WANG Yong-qin,. ConstRuction of RNAi ExpRessio VectoRs by Fusion Gene FRagment of TAV-CP，CVB-CP and CChMV[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(1): 106-111. doi: 10.3969/j.issn.1000-7091.2013.01.020.