Study on Physiological Traits of Drought Resistance for Transgenic Chrysanthemum Varieties Wanfen

doi:10.7668/hbnxb.2011.05.036

Abstract

Abstract: Transgenic chrysanthemum varieties Wanfen with OtMBY from Opisthopappus taihangensis,wide type Wanfen and Opisthopappus taihangensis were treated under water stress for 12 days,then re-watered.In the experiments,chlorophyll,soluble protein,malondianldehyde(MDA),proline,soluble sugar contents in leaves and relative permeability of cell membrane of leaves were determined.With the degree of drought stress increase,for all of the three,the content of chlorophyll in leaves decreased,while MDA,proline contents increased dramaticly.Compared with wild type Wanfen,However,the transgenic plants demonstrates greater stability and resistant,and showed similar physiological indicators variation to O.taihangensis,which its OtMYB came from.This indicated that OtMYB gene had expressed in the transgenic plants and improved their drought resistance.Moreover,the trend of soluble protein and soluble sugar contents in transformed chrysanthemum was similar to that in O.taihangensis,significantly different from that in wild type Wanfen,suggesting OtMYB gene probably could induce sugar and protein-specific expression under drought stress.

Key words: Transgenic, Ground-cover chrysanthemum, OtMYB, Drought resistance, Physiological characteristics

CLC Number:

S682.11

ZHANG Lu, ZHANG Qi-xiang, GAO Yi-ke, LU Miao, WANG Ya. Study on Physiological Traits of Drought Resistance for Transgenic Chrysanthemum Varieties Wanfen[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(5): 181-185. doi: 10.7668/hbnxb.2011.05.036.

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References

[1] Xu D,Duan X,Wang B,et al.Expression of a late embryogenesis abundant protein gene,HVAI,from barley confers tolerance to water deficit and salt stress in tramgenic rice[J].Plant Physiol,1996,110 (1):249-257.
[2] Quan R D,Hu S J,Zhang Z,et al.Overexpression of an ERF transcriptionfactor TSRFI improves rice drought tolerance[J].Plant Biotechnology Journal,2010,8 (4):476-488.
[3] Liangqi W,Zhanmin F,Lei G,et al.Over-expression of the bacterial nhaA gone in rice enhances salt and drought tolerance[J].Plant Science,2005,168:297-302.
[4] Cominelli E,Galbiati M,Vanvasseur A,et al.Aguard-cellspecific MYB transcription factor regulates sto metal movements and plant drought tolerance[J].Curr Biol,2005,15(13):1196-2000.
[5] Vannini C,Locatelli F,Bracale M,et al.Overexpression of the rice Osmyb4 gene increases chilling and freezing tolerance of Arabidopsis thaliana plants[J].Plant J,2004,37(1):115-127.
[6] Hoeren F U,Dolferus R,Wu Y,et al.Evidence for a role for AtMYB2 in the induction of the Arabidopsis alcohol dehydrogenase gene (ADH1) by low oxygen[J].Genetics,1998,149 (2):479-490.
[7] Gaxiola R A,Li J,Undurraga S,et al.Drought and salttolrant plants result from overexpression of the AVP1 H +-pump[J].Proc Natl Acad Sci USA,2001,98 (20):11444-11449.
[8] Van C W,Willekens H,Bowler C,et al.Elevated levels of superoxide dismutase protect transgenic plants against ozone damage[J].Bio/Technology,1994,12:165-168.
[9] Perl A,Perl-Treves R,Galili S,et al.Enhanced oxidativestress defense in transgenic potato expressing tomato Cu,Zn superoxide dismutases[J].Theor Appl Genet,1993,85:568-576.
[10] Ebskamp M J,van der Meer I M,Spronk B A,et al.Accumulation of fructose polymers in transgenic tobacco[J].Biotechnology (NY),1994,12 (3):272-375.
[11] 梁峥,马德钦,汤岚,等.菠菜甜菜碱醛脱氢酶基因在烟草中的表达[J].生物工程学报,1997,13(3):236-240.
[12] Dai X Y,Xu Y Y,Ma Q B,et al.Overexpression of a R1R2R3 MYB gene,OsMYB3R-2,increases tolerance to freezing,drought,and salt stress in transgenic Arabidopsis[J].Plant Physiology,2007,13 (4):1739-1751.
[13] Agarwal M,Hao Y,Kapoor A,et al.A R2R3 type MYB transcription factor is involved in the cold regulation of CBF genes and in acquired freezing tolerance[J].Journal of Biological Chemistry,2006,281 (49):37636-37645.
[14] 陆苗.太行菊抗旱相关基因的分离及其在菊花中的表达[D].北京:北京林业大学,2009.
[15] 李合生,孙群,赵世杰.植物生理生化试验原理和技术[M].北京:高等教育出版社,2000:120-136.
[16] Morgan J A.The effects of N nutrition on the water relations and gas exchange characteristics of wheat(Triticum aestivum L.)[J].Plant Physiology,1986,80(1):52-58.
[17] Singh T N,Aspinall D,Paleg L G.Proline accumulation and varietal adaptability to drought in barley:a potential metabolic measure of drought resistance[J].Nature New Biol,1972,236 (12):188-190.
[18] 周桂,李阳瑞.植物干旱诱导蛋白研究进展[J].广西农业科学,2007,38(4):379-385.
[19] Kishor P,Hong Z,Mine G H,et al.Overexpression of[delta]-Pyrro-line-5-Carboxyiato synthetase increases proime production and confers osmotolerance in transgenic plants[J].Plant Physiol,1995,108 (4):1387-1394.
[20] Liu J,Zhu J K.Proline accumulation and salt-stress-induced gone expression in a salt-hypersensitive mutant of Arabidopsis[J].Plant Physiol,1997,114 (2):591-596.
[21] Liu J,Huang S.Studies on high salt tolerance of transcripts encoding water channel proteins:tissue-specific expression in the common ice plant[J].Plant Cell,1995,7(8):1129-1142.
[22] Pilon-Smits E,Ebskamp M,Paul M J,et al.Improved performance of transgenic fructan-accumulating tobacco under drought stress[J].Plant Physiol,1995,107 (1):125-130.

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