Expression of the Capsid :Precursor Polypeptide P1 of Foot2and2mouth Disease Virus Type C and Analysis of Its Biology Activity

doi:10.7668/hbnxb.2009.05.002

Abstract

Abstract: The complete gene encoding the structural protein of FMDV (P1) was subcloned into expression vector pET228α(+),resulting in the fusion expression plasmid pET2P1.After transformed into E.coli (DE3) and induced by IPTG,the results of SDS2PAGE showed that the fusion protein was expressed in high level.The molecular weight of the fusion protein was 85 kDa and the expressed products were significant at inclusion body.Western blotting was performed to confirm that the expressed fusion protein could specifically react with antiserum against FMDV.The fusion protein were further purified and vaccined antigen based on the purified protein was developed.

Key words: FMDV type C, Recombiant P1, Expression biology acting

CLC Number:

Q785

CHANG Hui-yun, CONG Guo-zheng, DU Jun-zheng, SHAO Jun-jun, LIN Tong, FENG Jin-rui, LIU Ping. Expression of the Capsid :Precursor Polypeptide P1 of Foot2and2mouth Disease Virus Type C and Analysis of Its Biology Activity[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(5): 7-10. doi: 10.7668/hbnxb.2009.05.002.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2009.05.002

http://www.hbnxb.net/EN/Y2009/V24/I5/7

References

[1] Saiz M,Nune J I,Jimenez M.Foot-and-mouth disease virus biology and prospects for disease control[J].Microbes Infect,2002,4(1):1103-1192.
[2] Ling Jie-yu,Liu Zhao,Tong Tie-zhu.Construction and eukaryotic expression of recombinant plasmid encoding fusion protein of goat complement C3d and foot and mouth disease virus VP1[J].Chinese Journal of Biotechnology,2008,24(2):209-213.
[3] 周建华,丛国正,高闪电.口蹄疫病毒株OA/58L蛋白酶的结构构建和功能分析[J].华北农学报,2007,22(4):176-179.
[4] Li Yan-min,Catrina M A,Stirling Michael S Denyer.Dramatic improvement in FMD DNA vaccine efficacy and cross-serotype antibody induction in pigs following a protein boost[J].Vaccine,2008,26:2647-2656.
[5] Balamurugan V,Renji R,Ana S N,et al.Protective immune response of the capsid prectorsor polypetide(P1) of foot and mouth disease virus type O produced in pichina postors[J].Vaccine,2003,92:141-149.
[6] 潘丽,张永光,王永录,等.口蹄疫结构蛋白P1-2A及蛋白酶3C基因的转基因番茄对豚鼠免疫反应的初步研究[J].病毒学报,2006,22(4):297-303.
[7] Sanz Parra A,Jimenez Clavero M A,Garc Briones M M,et al.Recombinant viruse expression the foot-and-mouth disease virus capsid precursur polypetide P1 induce celluiar or not humoral antiviral immunity and partial protection in pigs[J].Virology,1999,259:129-134.
[8] 余晓岚,肖少波,方六荣,等.口蹄疫病毒P1基因在大肠杆菌中的高效表达及其生物活性的初步分析[J].生物工程学报,2005,21(1):163-167.
[9] Sanz A,Vazquez B,Sobrino F,et al.Evidence of partial protection against foot-and-mouth disease in cattle immunized with a recombinant adenovirus vector expressing the precursor polyeptide(P1)of foot-and-mouth disease virus capsid protein[J].Gen Virol,1999,80(3):671-679.
[10] 周建华,丛国正,高闪电,等.口蹄疫OA/58病毒株CP2蛋白结构模拟与B细肥抗原原表位的分析[J].华北农学报,2008,23(3):5-8.
[11] Berinstein A,Taboga,Smitsaart E,et al.Protective immunity against foot-and-mouth disease viruse induced by a recombint vaccinia virus[J].Vaccine,2000,18:2231-2238.

[1]	WANG Xiaojing, SUN Linjing, SUN Yue, ZHANG Rongxue, LI Junling, YAN Shuangyong, SU Jingping. *Construction of Plant Binary Expression Vector for OsPIP2; 7* with HA-tag and Its Transformation in Rice** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(5): 59-64.
[2]	LIU Yufeng, ZHU Tianhui, LIU Yinggao, QIAO Tianmin, LI Shujiang, LONG Xumei, HAN Shan. *Cloning, Sequence Analysis and Prokaryotic Expression of CmWRKY* from Castanea mollissima BL** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(4): 37-45.
[3]	GUO Yue, JIANG Pingping, PAN Leilei, ZHOU Wenjie, XU Lei, ZHANG Ruqin, SUI Jiongming, GUO Baotai, WANG Jingshan, QIAO Lixian. Cloning and Disease Resistance Verification of Chitinase Gene in Peanut [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(6): 42-48.
[4]	YAN Wenfei, CHENG Fansheng, JIANG Xinqiang, LIU Cuixia, ZHU Dan. Cloning and Expression Analysis of GsTIFY6B Associated with Saline and Alkali Stress in Glycine soja [J]. Acta Agriculturae Boreali-Sinica, 2018, 33(4): 82-89.
[5]	WANG Junbin, YANG Wenli, DING Bo, WU Tianwen, WANG Haifeng, XIE Xiaodong. *Cloning,Bioinformatics and Expression Analysis of TaWRKY71a* Gene in Wheat** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(3): 7-13.
[6]	CHEN Guanxu, QIN Guilong, LI Enguang, ZHAO Chunmei, QIAO Lixian, WANG Jingshan, SUI Jiongming. *Identification and Salinity Stress-responsive Analysis of PP2C* Genes in Peanut** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(3): 71-77.
[7]	WANG Dan, XIE Wenjie, ZHU Yefei, ZHAO Jinliang, CHEN Xiaowu. Expression Characterization of Interferon Regulatory Factor 1 in Nile Tilapia [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(4): 31-38.
[8]	CHENG Ye, YAO Daxuan, LIU Yunting, HU Wenjing, DUAN Huijun. *Cloning Flanking Sequences Sweet Corn Mutant of Maize Inserted by Mutator* Transposon** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(2): 28-31.
[9]	YAN Shu-feng, TIE Shuang-gui, YUE Run-qing, HAN Xiao-hua, QI Jian-shuang, LU Cai-xia. *Acquirement and Molecular Identification of Transgenic Insect-resistant Corn Expressing Cry1Ac-w* Gene** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(5): 41-44.
[10]	SUN Bing-bing, ZHANG Wei, DUAN Hong-ying, LI Wei, TIAN Tao, ZHANG Li-qun. *Cloning of EnvZ/OmpR Two-component System from Pseudomonas fluorescens* 2P24 and Prokaryotic Expression of OmpR** [J]. journal1, 2014, 29(3): 41-45.
[11]	XU Pin-san, SUN Bing-lun, JIANG Xu, LI Huan-gai. Lily Mottle Virus Genome of Dalian Isolates Sequencing and Its Analysis [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(S1): 12-15.
[12]	YU Yue-hua,gENG Hong-wei, CHEN Quan-jia,gAO Wen-wei, WANG Li-ping, QU Yan-ying. *Prokaryotic Expression Analysis of Transcription Factor TaSIM* from Triticum aestivum L． YU Yue-hua，gENG Hong-wei， CHEN Quan-jia，gAO Wen-wei， WANG** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(2): 60-62.
[13]	LIN Shi-feng, REN Xue-liang, WANG Yi, WANG Dong-mao, ZHANG Tuo, WANG Ren-gang. Cloning and Sequence Analysis of Three Betaine Aldehyde Dehydrogenase Genes in Tobacco(Nicotiana tabacum ) [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 17-22.
[14]	LI Meng, ZHANG Ai-hua, BAI Xue-fei, WANG Cui-xia, DUAN Hui-jun. Silico Cloning and Bioinformatics Analysis of Zeaxanthin Epoxidase Gene(ZE) in Cucunis melo [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(S1): 23-28.
[15]	REN Lei, WANG Yan, ZHOU Lin, PENG Zhen-hua. *Cloning,Expression and Vector Construction of PsMDH* Gene in Tree Peony** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(4): 32-37.

Please choose a citation manager

Content to export