Silico Cloning and Bioinformatics Analysis of Zeaxanthin Epoxidase Gene(ZE) in Cucunis melo

doi:10.7668/hbnxb.2011.S1.005

Abstract

Abstract: In silico cloning and function prediction of Zeaxanthin epoxidase gene(ZE) is researched in the paper.Zeaxanthin epoxidase gene was cloning by the EST databases and the EST assembly tools,CAP3 based on the principal that orthologue genes have the alike function.Using the bioinformatical databases and softwares,its function was analyzed and predicted.The results showed that the cDNA length of ZE is 2 323 bp long and a single open reading frame encodes 657 amino acid residues with calculated molecular mass of 72.4 kDa and a conserved domain of FHA composed of 56 amino acid(553-608).ZE protein is a stable hydrophilic protein and taking part in the xanthophyll cycle,carotenoid biosynthesis metabolic reactions.

Key words: Zeaxanthin epoxidase, Silico cloning, Bioinformatics

CLC Number:

Q785

LI Meng, ZHANG Ai-hua, BAI Xue-fei, WANG Cui-xia, DUAN Hui-jun. Silico Cloning and Bioinformatics Analysis of Zeaxanthin Epoxidase Gene(ZE) in Cucunis melo[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(S1): 23-28. doi: 10.7668/hbnxb.2011.S1.005.

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