Cloning,Expression and Vector Construction of PsMDH Gene in Tree Peony

doi:10.7668/hbnxb.2011.04.005

Abstract

Abstract: In this work,a full-length cDNA sequence of MDH gene was obtained from petals of tree peony(Paeonia suffruticosa L.cv.Zhao Fen)using RT-PCR and RACE,named PsMDH(GenBank accession No.HQ449567).The full length of PsMDH cDNA is 1 283 bp,containing a 5′-untranslated region(5′-UTR) of 80 bp,a 3′-UTR of 203 bp,and an opening reading frame(ORF) of 999 bp encoding a 332 predicted amino acids.Subsequently,sequence comparison and phylogenetic analysis revealed that PsMDH shared more than 93.37% homology with Populus trichocarpa.Relative Real-time PCR analysis indicated that PsMDH showed the highest transcript abundance in carpels and the lowest levels in stamens.Construction of plant expression vector lay a foundation for variety improvement in tree peony.

Key words: Tree peony, Cloning of malate dehydrogenases, Bioinformatics analysis , Expression , Construction of vector

CLC Number:

Q785

REN Lei, WANG Yan, ZHOU Lin, PENG Zhen-hua. Cloning,Expression and Vector Construction of PsMDH Gene in Tree Peony[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(4): 32-37. doi: 10.7668/hbnxb.2011.04.005.

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