Expression Characterization of Interferon Regulatory Factor 1 in Nile Tilapia

doi:10.7668/hbnxb.2016.04.006

Abstract

Abstract: In order to study the expression and function of interferon Regulatory Factor (IRF1) in nile tilapia,the full length cDNA of IRF1 was cloned and applied for phylogenetic analysis,and the expression variation was checked by qRT-PCR after PolyI:C stimulation.Cell transfection by eukaryotic expression vector was used to analyzing for subcellular localization.The opening reading frame of nile tilapia IRF1 contains 888 bp with deduced protein of 295 amino acids.Structurally,nile tilapia IRF1 protein typically shares the conserved characterizations with other species' IRF1 homologues,displaying conserved DNA-binding domain,IRF association domain,serine-rich C terminal domain,and 6 tryptophan residues in the N terminal.The results of phylogenetic analysis showed that the IRF family was divided into four subfamily:IRF1,IRF3,IRF4 and IRF5.The nile tilapia IRF1 was found to be merged into the teleost subgroup,amphibian and mammalian homologues were clustered into their corresponding subgroups.Real-time PCR revealed a broad expression pattern of nile tilapia IRF1 in various tissues.The expression of IRF1 was mainly in the head kidney,spleen,liver and gill,furthermore,which increased obviously after PolyI:C stimulation,especially for the spleen and head kidney.Subcellular localization analysis showed that nile tilapia IRF1 mainly resides in the cytoplasm.These data supported the view that nile tilapia IRF1 was a potential molecule in IFN immune defense system against viral infection.

Key words: Interferon regulatory factor, Nile tilapia, Phyletic evolution, Subcellular localization

CLC Number:

Q785
S917

WANG Dan, XIE Wenjie, ZHU Yefei, ZHAO Jinliang, CHEN Xiaowu. Expression Characterization of Interferon Regulatory Factor 1 in Nile Tilapia[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(4): 31-38. doi: 10.7668/hbnxb.2016.04.006.

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