Identification of Differential Proteins in Roots of Tobacco with Overexpressing TaZAT8, a Zinc Finger Type Transcription Factor Gene from Wheat

doi:10.7668/hbnxb.201751771

Abstract

Abstract: In order to further clarify the mechanism of over expression TaZAT8, a wheat C2H2 type zinc finger gene, to enhance the resistance of tobacco plants under P-starvation, the classical proteomics approach was applied to reveal the changes of protein expression between WT and transgenic tobacco plants. The results showed that there were 22 protein spots differentially expressed on 2-DE images, and among them, twenty protein spots were up-regulated and two were down-regulated respectively. Subsequently, these protein spots were identified by LC-MS/MS, which were classified into six functional groups, including primary metabolism, energy generation, stress response and defense, transcription and translation, protein turnover and unclear functions. Subcellular localization analysis indicated that these proteins were mainly located in five organelles, including cytoplasm, mitochondria, peroxisome, nucleus and chloroplast. Go analysis showed that the differentially expressed proteins participated in various metabolism processes or act as enzymes of different categories. Above results showed that over expression TaZAT8 laid the foundation for improving the plant tolerance to stress via regulating proteins involved in biological processes such as substance metabolism, energy production, stress response, etc.

Key words: Tobacco root, Overexpressing TaZAT8, Proteome, Subcellular localization, GO analysis

CLC Number:

Q78
S572

GU Qian, DING Weiwei, JIANG Mingyue, SU Xiaoshuai, LI Xiaojuan, XIAO Kai. Identification of Differential Proteins in Roots of Tobacco with Overexpressing TaZAT8, a Zinc Finger Type Transcription Factor Gene from Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(5): 45-51. doi: 10.7668/hbnxb.201751771.

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