Genetic Mapping and QTL Analysis of Fruit Russeting in Malus domestica Borkh.

doi:10.7668/hbnxb.201751695

Abstract

Abstract: In order to effectively discover the apple fruit russet gene, based on SLAF-seq technology, the genetic map construction and QTL analysis for fruit russet gene were conducted using a population derived from a cross between Miyazaki Spur and Sakata Tsugaru. The results showed that 522 122 315 reads (110.32 Gb) of the sequencing data from seedlings was obtained, in which the average Q30 sequencing was 95.07%, and the average GC content and its parents was 40.11%. Then, 20 440 SLAF markers and 7 309 729 SNPs were developed. Based on these markers, seventeen linkage groups were constructed, of which 4 075 markers presented in the genetic map, and marker integrity was 99.92%. The total diagram spacing was 2 235.23 cM, and the average diagram spacing was 0.55 cM. A total of 9 QTLs related to fruit russet were detected, which were distributed on Chr3, Chr9, Chr11, and Chr15 chromosomes, with a marker distance of 0-4.9 cM and a contribution rate of 18.0%-85.5%. Among them, Qru-9, Qru-15 and Qru-3, located on Chromosome 9, 3, and 15, respectively, might be novel QTLs for fruit russeting of apple. A genetic map based on SLAF-seq method was constructed in apple progeny Miyazaki Spur crossing Sakata Tsugaru and nine QTLs related to apple russeting were obtained. These results provided the important information for further exploration of russeting QTL in apple.

CLC Number:

S661.03

ZHANG Chaohong, CHEN Dongmei, YANG Fengqiu, ZHAO Tongsheng, LI Yang, ZHAO Guodong, ZHAO Yongbo. Genetic Mapping and QTL Analysis of Fruit Russeting in Malus domestica Borkh.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(5): 37-44. doi: 10.7668/hbnxb.201751695.

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