Cloning and Expression Analysis of Four Apple MdCBF Genes

doi:10.7668/hbnxb.2017.S1.004

Abstract

Abstract: In order to select antifreeze negative gene in apple, lay a foundation for cultivating new varieties of antifreeze apples,4 open reading frame CBF genes were cloned from apple by the method of information biology,named as MdCBFL1-MdCBFL4. These genes(MdCBFL1-MdCBFL4) were shown to encode proteins that share a high similarity with plant cold stress-related CBF proteins,which contained a AP2 domain and two classic sequences that conserved PKRPAGRTKFRETRHP nuclear location signal area and DSAWR conservative sequence. Phylogenetic analysis revealed that the isolated apple genes could be classified into the Arabidopsis A-1 subgroup, it was speculated that it was similar to the function of Arabidopsis A-1 transcription factor, and the expression of MdCBFL1-MdCBFL4 proteins was induced by stress of drought, low temperature and salt stress.The expression of MdCBFL1-MdCBFL4 gene in apple was detected by semi-quantitative RT-PCR in high salt(200 mmol/L NaCl),drought and low temperature.The results showed that MdCBFL1 gene was response to the low temperature,drought and salt stress. MdCBFL2-MdCBFL4 gene was induced by low temperature and high salt. This provides useful clues to further elucidate the function and mechanism of apple MdCBF gene in stress response.

Key words: Apple, CBF/DREB protein, Gene cloning, Abiotic stress, Analysis of gene expression

CLC Number:

Q78
S661.03

JIN Qin, DAI Peihong, LIU Chao, HU Qinhua, LIU Xiaodong, LI Yue. Cloning and Expression Analysis of Four Apple MdCBF Genes[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(S1): 17-22. doi: 10.7668/hbnxb.2017.S1.004.

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