Antimicrobial Susceptibility and SCCmec Genotyping of Methicillin Staphylococcus aureus Isolates from Swine in Sichuan

doi:10.7668/hbnxb.2017.S1.005

Abstract

Abstract: To study the drug resistance of SCCmec resistant Staphylococcus aureus and analyze pubic health in Sichuan,we collected 120 samples from Sichuan pig-farms to identify MRSA by PCR test from Sichuan. Then identified these 22 MRSA by drug-resistance testing and SCCmec typing.In these samples,60 strains of Staphylococcus aureus had been isolated from pig water and the hand of worker,including 22 MRSA,the detection rate was 18.3% (22/120). Susceptibility testing results showed that this 22 multi-drug resistant MRSA strains had significant multi-drug resistance,most were 4,6-resistant. Genotyping results showed that the predominant type of the Sichuan MRSA were mainly SCCmec Ⅳ a type,including 17 strains of SCCmec Ⅳ a type and 5 strains of SCCmec Ⅰ type.Last discuss the MRSA from swine have imperiled the health of worker and public health。

Key words: Methicillin-resistantst Staphylococcus aureus, SCCmec, Typing drug sensitivity test, Public health

CLC Number:

Q78
S442

XU Siyao, HUANG Pu, LI Ping, YANG Fan, JIANG Ziyi, LÜ Wenting, XU Zhiwen, ZHU Ling. Antimicrobial Susceptibility and SCCmec Genotyping of Methicillin Staphylococcus aureus Isolates from Swine in Sichuan[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(S1): 23-29. doi: 10.7668/hbnxb.2017.S1.005.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2017.S1.005

http://www.hbnxb.net/EN/Y2017/V32/IS1/23

References

[1] Vyas K,Hospenthal D R,Mende K,et al. Recurrent community-acquired methicillin-resistant Staphylococcus aureus infections in an HIV-infected person[J]. Journal of Clinical Microbiology,2011,49(5):2047-2053.
[2] 战晓微,郑秋月.耐甲氧西林金黄色葡萄球菌耐药性及SCCmec基因分型研究[J].国际检验医学杂志,2014,35(18):2512-2514.
[3] Khanna T,Friendship R,Dewey C,et al. Methicillin resistant Staphylococcus aureus colonization in pigs and pig farmers[J]. Veterinary Microbiology,2008,128(3/4):298-303.
[4] 程建平,曾浩,邹全明,等.耐甲氧西林金黄色葡萄球菌基因诊断及多重PCR SCCmec基因分型方法的建立[J].中华医院感染学杂志,2012,22(11):2244-2247.
[5] Zhang K,Mcclure J A,Elsayed S,et al. Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types Ⅰ to Ⅴ in methicillin-resistant Staphylococcus aureus[J]. Journal of Clinical Microbiology,2005,43(10):5026-5033.
[6] Hiramatsu K,Ito T,Tsubakishita S,et al. Genomic basis for methicillin resistance in Staphylococcus aureus[J]. Infection & chemotherapy,2013,45(2):117-136.
[7] 师志云,赵志军,贾伟,等.耐甲氧西林金黄色葡萄球菌分子流行病学研究[J].中华医院感染学杂志,2011,21(14):2886-2888.
[8] Huijsdens X W,Van Dijke B J,Spalburg E,et al. Community-acquired MRSA and pig-farming[J]. Annals of Clinical Microbiology and Antimicrobials,2006,5(1):26.
[9] Cui S,Li J,Hu C,et al. Isolation and characterization of methicillin-resistant Staphylococcus aureus from swine and workers in China[J]. The Journal of Antimicrobial Chemotherapy,2009,64(4):680-683.
[10] Guardabassi L,O'donoghue M,Moodley A,et al. Novel lineage of methicillin-resistant Staphylococcus aureus,Hong Kong[J]. Emerging Infectious Diseases,2009,15(12):1998-2000.
[11] Yan X,Yu X,Tao X,et al. Staphylococcus aureus ST398 from slaughter pigs in Northeast China[J]. International Journal of Medical Microbiology,2014,304(3/4):379-383.
[12] 樊润,吴聪明,李德喜,等.河南省猪源耐甲氧西林金黄色葡萄球菌的耐药性及分子分型研究[J].中国兽医科学,2014(12):1223-1230.
[13] Wang X,Meng J,Zhou T,et al. Antimicrobial susceptibility testing and genotypic characterization of Staphylococcus aureus from food and food animals[J]. Foodborne Pathogens and Disease,2012,9(2):95-101.
[14] 王雪敏,姚建楠,李蓓蓓,等.上海市猪源与人源耐甲氧西林金黄色葡萄球菌的分离及分子分型[J].中国兽医科学,2013(3):252-255.
[15] Kraker M D,Jarlier V,Monen J M,et al. The changing epidemiology of bacteraemias in Europe:trends from the European antimicrobial resistance surveillance system[J]. Clinical Microbiology & Infection the Official Publication of the European Society of Clinical Microbiology & Infectious Diseases,2013,19(9):860-868.
[16] Peng Y,Ou Q,Lin D,et al. Metro system in Guangzhou as a hazardous reservoir of methicillin-resistant staphylococci:findings from a point-prevalence molecular epidemiologic study[J]. Scientific Reports,2015,5:16087.
[17] 纪风兵,邹自英,胡章勇,等.成都地区耐甲氧西林金黄色葡萄球菌SCCmec基因分型的研究[J].成都:四川医学,2016,37(4):364-368.
[18] 王新,黄山,周婷,等.猪源耐甲氧西林金黄色葡萄球菌的耐药性及其SCCmec基因分型研究[J].中国预防兽医学报,2010,32(12):975-977.
[19] 张泓,李万华,蔡琴,等.MRSA杀白细胞素基因检测和SCCmec分子流行病学调查[J].中国感染与化疗杂志,2009,9(4):248-251.
[20] 黄仆.四川地区猪群中耐甲氧西林金黄色葡萄球菌的分离鉴定和耐药性研究[D].成都:四川农业大学,2014.

Please choose a citation manager

Content to export