Analysis of Resistance Gene Analogues from the Section of Mildew Powdery Resistance Gene Pm43 in Wheat

doi:10.7668/hbnxb.2016.04.005

Abstract

Abstract: Draft sequencing data of common wheat can be used to analysis a target section of single chromosome from genome-wide in wheat.A powdery mildew resistance gene,Pm43,was assigned on the long arm of wheat 2D chromosome by Shanxi Key Laboratory of Crop Genetics and Molecular Improvement.Bioinformatics methods were used to determine the position of Pm43 in the physical map,genetic map and genome map in wheat.The results provided a reference to the fine mapping or the candidate genes determining ofPm43.By aligning the sequences of flanking markers,the Pm43 was mapped on the 79-99 cM of C-2DL3-0.49 section,which was between 2DL_9835990 and 2DL_9823315 in genomic region.Using the conserved motifs of cloned resistance genes in wheat as a probe,89 RGA (Resistance gene analogues)-scaffolds were retrieved from the target region,and 36 scaffolds were containing SSR loci.Then SSR markers were developed.Applying the resistant (R) parent CH5025 carried Pm43,the susceptible (S) parent Taichang 29 and two R/S bulks of F₂ population crossed by CH5025 and Taichang 29,the linkage of developed SSR markers and Pm43 was detected.Four polymorphic markers were screened and further defined the target region between PK_9908430 and NBS_9908778.Then by cluster analysis,one PK sequence and one NBS sequence which had the highest homology with cloned Pm were screened,and their homologous RGA expressed sequences were found from Aegilopstauschii and rice genome.

Key words: Pm43, Wheat genome, Chromosome 2D, PK, NBS

CLC Number:

LIU Jing, QIAO Linyi, ZHANG Xiaojun, LI Xin, ZHAN Haixian, GUO Huijuan, ZHANG Xiaohui, FENG Jianning, CHANG Zhijian. Analysis of Resistance Gene Analogues from the Section of Mildew Powdery Resistance Gene Pm43 in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(4): 26-30. doi: 10.7668/hbnxb.2016.04.005.

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