Screening of Wheat NBS Genes in Response to Powdery Mildew

doi:10.7668/hbnxb.20192379

Abstract

Abstract: Nucleotide binding site(NBS) genes are a type of disease resistance genes widely present in plants.In order to screen the powdery mildew-resistant genes for wheat breeding,the changes in the expression level of wheat NBS genes after infection with Blumeria graminis f.sp.tritici(Bgt) were analyzed.Bioinformatics methods were used to assemble the original transcriptome data of a disease-resistant wheat variety infected by Bgt at 0,24,48,72 h,and 1 283 TaNBS with expression data were screened out,which were distributed on 21 chromosomes.Results of expression difference analysis showed that the expression levels of 395 TaNBS were significantly changed after being infected by Bgt,and they were divided into five types,including down-up-up,up-up-down,up-down-up,down-down-up as well as up-up-up,according to the change trend in three time periods(0-24 h,24-48 h,48-72 h).Using qRT-PCR technology,ten TaNBS with the most significant differences were selected from the up-up-up type for verification.The results showed that three genes Ta7dlLoc004854, Ta7dlLoc000139 and Ta3asLoc007663 remained significantly up-regulated in a self-breeding disease-resistant wheat germplasm CH7124 after being infected by Bgt. Using virus-induced gene silencing technology,the expression levels of the above three genes in CH7124 were down-regulated to obtain the silent plants.The results of the inoculation test showed that only the Bgt-infection type of Ta7dlLoc000139-silent plants changed from immune to high resistance or medium resistance,indicating that the decrease in the expression level of Ta7dlLoc000139 caused the plant resistance to weaken.Sequence analysis results showed that Ta7dlLoc000139 had a total length of 4 042 bp,contained three exons and two introns,and contained a large number of regulatory elements such as CGCG-Box,GATA-Box and CAAT-Box in the 2 000 bp region before its start codon.The research results could provide a theoretical basis for wheat disease resistance molecular breeding.

Key words: Wheat, NBS genes, Bgt response, Expression level, Gene silencing

CLC Number:

TIAN Zhigang, ZHANG Shuwei, CHEN Fang, CHANG Lifang, JIA Juqing, ZHANG Xiaojun, LI Xin. Screening of Wheat NBS Genes in Response to Powdery Mildew[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 189-194. doi: 10.7668/hbnxb.20192379.

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