Cloning and Expression of ZmPP2C6-01 Gene in Maize

doi:10.7668/hbnxb.201751477

Abstract

Abstract: In order to study the response of maize serine/threonine protein phosphatase PP2C gene to abiotic stress and its physiological function, the transcriptome of maize inbred line Yu 882 was analyzed under 20% PEG6000 stress. The PP2C gene strongly expressed under drought stress was screened and cloned, while the bioinformatics analysis and expression pattern analysis were carried out. A PP2C protein gene that was up-regulated significantly by drought stress was found in the 20% PEG6000 stress-treated transcriptome of maize, and was named as ZmPP2C6-01. The open reading frame of ZmPP2C6-01 gene was 1 242 bp, encoding 413 amino acids. The encoded protein had a molecular mass of 43.8 ku and an isoelectric point of 6.6. It was a hydrophilic protein. The ZmPP2C6-01 protein was closely related to the PP2C protein of sorghum, and also had the close relationship with the PP2C protein of winter wheat and Aegilops tauschii. The ZmPP2C6-01 protein was localized in the nucleus. Real-time quantitative PCR analysis showed that ZmPP2C6-01 gene had the highest expression in maize roots, followed by leaves and stems. Under the condition of PEG stress, the expression level of ZmPP2C6-01 gene in roots was higher than that of the control in most of the time, and it was up-regulated in leaves. However, under the stresses of ABA, NaCl and high temperature, the expression level of ZmPP2C6-01 gene in roots was lower than that of the control, and the overall expression showed a downward trend, while the expression level in leaves was higher than that of the control, showing an up-regulated expression pattern. In summary, the ZmPP2C6-01 gene responded to drought and other stresses, but the expression patterns were inconsistent.

Key words: Zea mays, ZmPP2C6-01, Subcellular localization, Abiotic stress, Expression analysis

CLC Number:

S513.03
Q78

YUAN Zhen, ZHANG Pengyu, WANG Guorui, CAO Liru, KU Lixia, WEI Li. Cloning and Expression of ZmPP2C6-01 Gene in Maize[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(2): 8-18. doi: 10.7668/hbnxb.201751477.

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