Molecular Characterization and Target Gene Prediction of Plant miR399 Family

doi:10.7668/hbnxb.201751113

Abstract

Abstract: MicroRNA plays a variety of important regulatory roles in cells. The miR399 family plays a role in plant response to phosphorus starvation stress. In order to further understand the composition, molecular characteristics and target gene function of plant miR399 family, the number, phylogeny, secondary structure, promoter characteristics and target genes of miR399 family were analyzed by bioinformatics methods. Results showed that a total of 244 mature miR399 members were obtained, distributing only in angiosperms. The distribution of pre-miR399 and mature miR399 in different species varied greatly. Most pre-miR399 was only cut into a single mature miR399. The pre-miR399 with the largest number was 64 nt, and 93.85% of mature miR399 was only 21 nt. Pre-miR399 sequence analysis showed that 3' end was more conservative than 5' end. Most of the mature miR399 sequence was derived from the 3' end of pre-miR399, and the conservative sequence was UGCCAAAGGAGA*UUGCCC*G. Evolutionary analysis showed that the miR399 sequence was more consistent, but the cluster relationship of different species was complex. pre-miR399 was mainly divided into three types:①Producing one mature miR399 from 3' end;②Producing two mature miR399 respectively from 3' and 5' end; ③Producing one mature miR399 from 5' end. The pre-miR399 promoter region contained a large number of hormones and stress response elements. Gene ontology analysis of the target genes in rice miR399 family showed that the proportion of the cell and the cell part classifications were 59.1%, while the proportion of the catalytic activity and the binding types were 40.9% and 45.5% respectively. The miR399 regulated multiple phosphorus response and phosphorus transport target genes. Those target genes are mainly involved in plant phosphorus metabolism, stress response, gene expression regulation and many other processes. Above results indicated that miR399 was highly conserved and participated many signal processes, and played a role in plant phosphorus starvation stress and phosphorus transport.

Key words: miR399, Evolution, Target genes, Phosphorus stress

CLC Number:

Q789

LIU Chao, CHU Honglong, HAN Lihong, DAI Dongqin, CHEN Huanhuan, TANG Lizhou. Molecular Characterization and Target Gene Prediction of Plant miR399 Family[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(2): 1-7. doi: 10.7668/hbnxb.201751113.

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