Isolation and Identification of One Bacterium Species with High Effective Ability of Removing Ammonia-nitrogen

doi:10.7668/hbnxb.2014.04.038

Abstract

Abstract: Microbial method was used to remove ammonia-nitrogen pollutant from the aquaculture water,isolated 31 strains with high Ammonia-nitrogen removing ability from the aquaculture sediment.Among these strains,strain AD-8 showed the highest ability of removing ammonia-nitrogen.In the culture,its removal rate to 200 mg/L ammonia nitrogen was 86%,its removal rate was 91.2% in 48 h in low temperature(15℃).In simulated aquaculture waste water,its net removal efficiency of ammonia nitrogen was 90% after 48 hours.AD-8 was identified as Pseudomonas alcaliphila according to its morphological,physiological and biochemical properties and phylogenetic analysis of its 16S rDNA sequence.

Key words: Ammonia nitrogen removal ability, Isolation and identification, 16S rDNA, Pseudomonas alcaliphila

CLC Number:

X172
X703

ZHANG Feng-feng, XIE Feng-xing, ZHOU Ke, SUN Hai-bo, ZHAO Yu-jie. Isolation and Identification of One Bacterium Species with High Effective Ability of Removing Ammonia-nitrogen[J]. journal1, 2014, 29(4): 221-225. doi: 10.7668/hbnxb.2014.04.038.

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References

[1] 刘淑集,吴成业.水产饲用益生菌的作用机理及功效[J].天津农业科学,2010,16(5):58-62.
[2] 臧淑梅.氨氮在水产养殖中的产生、危害及控制[J].黑龙江水产,2012(2):38-39.
[3] 高宇,刘文斌,王莹,等.可用于水体污染控制的氨氮转化菌筛选及部分降解特性的实验研究[J].环境工程学报,2010,4(4):855-860.
[4] 符吴萸,杜丽明,杨平,等.养殖水体氨氮降解菌的分离与降解特性研究[J].广东石油化工学院学报,2013,23(4):22-26.
[5] 张峰峰,谢凤行,赵玉洁,等.一株反硝化细菌的分离鉴定及脱氮能力[J].环境工程学报,2013,4(11):4415-4420.
[6] 国家环境保护总局编委会.水和废水检测分析方法[M].4版.北京:中国环境科学出版社,2002:268-281.
[7] 东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001:180-181.
[8] 易弋,容元平,程谦伟,等.养殖水体氨氮降解菌的分离和初步鉴定[J].贵州农业科学,2011,3(2):154-157.
[9] 侯颖,孙军德,徐建强,等.巨大芽孢杆菌对养殖水体氨氮降解特性研究[J].沈阳农业大学学报,2006,37(4):607-610.
[10] 王娟,戴习林,宋增福,等.一株氨化细菌的分离、鉴定及氨氮降解能力的初步分析[J].水生生物学报,2010,34(6):1198-1201.
[11] 张磊,缪鑫昕,谈曙明,等.高氨氮降解菌株 DA-1 的分离鉴定及降解性能研究[J].江西农业学报,2012,24(3):148-150.
[12] 陈昢圳.畜禽养殖废水高效脱氮降解菌的筛选及脱氮性能研究[D].兰州:甘肃农业大学,2009.
[13] 周迎芹,信欣,姚力,等.一株高效异养硝化-好氧反硝化菌的分离鉴定及脱氮性能[J].环境工程学报,2013,7(10):4127-4132.
[14] 陈茂霞,王欢,周后珍,等.异养硝化-好氧反硝化菌HN-02的筛选及其特性[J].应用与环境生物学报,2013,19(4):688-693.
[15] Moir J B,Crossman L C,Spiro S,et al.The purification of ammonia monooxygenase from Paracoccuus denitrificans [J].FEBS Letters,1996,387(1):71-74.
[16] Joo H S,Hirai M,Shoda M.Piggery wastewater treatment using Alcaligenes faecalis strain No.4 with heterotrophic nitrification and aerobic denitrification[J].Water Research,2006,40(16):3029-3036.
[17] Blagodatsky S A,Kesik M,Papen H,et al.Production of NO and N₂O by the heterotrophic nitrifier Alcaligenes faecalis parafaecalis under varying conditions of oxygen saturation[J].Geomicrobiology Journal,2006,23(314):165-176.
[18] 张光亚,陈美慈,韩如旸,等.一株异养硝化细菌的分离及系统发育分析[J].微生物学报,2003,43(2):156-161.

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