Illumina Amplicon Sequencing of 16S rDNA Tag Reveals Bacteria Community Development of Long-term Straw Returning Soil

doi:10.7668/hbnxb.2016.06.025

Abstract

Abstract: In order to explore the influence of the typical long term orientation of straw returning to field on soil microbial diversity and the relationship between soil microbial diversity and ecological environment effect, using 16S rDNA PCR sequencing technology,continuous 6 years of long term positioning of straw to field test,analysis of the composition of soil bacterial community structure.The results showed that soil microbial community diversity and species richness could be promoted after long-term fertilization of straw and organic fertilizer.The dominant species of bacteria in the soil after straw returning to field were the deformation of the Proteobacteria and Acidobacteria.Principal component analysis that significant differences in the types and contents of microorganisms among different treatments.WC and WCN treatment of N, P, K and other available nutrient content was significantly different.WCN treatment in the N, P, K and other available nutrients, organic carbon content an d invertase, urease, cellulase activity was significantly higher than WC.The application of nitrogen fertilizer could significantly increase the soil nutrient content and enhance the soil enzyme activity, conducive to the improvement of soil is bacterial community diversity and stability, and improve the ecological environment of soil, so as to promote the increase of crop yield.

Key words: Straw returning, 16S rDNA, Long-term experiment, Soil bacterial community

CLC Number:

S158

YUAN Mingzhang, LIU Shutang, CHEN Yanling, XIN Li, LIU Jintao. Illumina Amplicon Sequencing of 16S rDNA Tag Reveals Bacteria Community Development of Long-term Straw Returning Soil[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(6): 157-163. doi: 10.7668/hbnxb.2016.06.025.

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